Nyberg S L, Shatford R A, Payne W D, Hu W S, Cerra F B
Department of Surgery, University of Minnesota, Minneapolis 55455.
Cytotechnology. 1992;10(3):205-15. doi: 10.1007/BF00146671.
A static culture model employing cylindrical collagen-hepatocyte gels is reported for large scale testing of conditions relevant to the three compartment hollow fiber bioartificial liver. High density hepatocyte cultivation was achieved by cell entrapment within the collagen-hepatocyte gel. Hepatocyte viability was assessed by vital staining, gel contraction, and insulin utilization. Measures of hepatocyte-specific function included albumin synthesis, ureagenesis, lidocaine biotransformation, and cholate conjugation. Although hepatocyte viability remained stable through the seven day incubation period, hepatocyte functions were not uniformly preserved. Albumin synthesis remained stable, while representative P-450 and conjugation activities decreased with time. This static culture system will facilitate the development of a hollow fiber bioartificial liver which utilizes cylindrical collagen-hepatocyte gels.
报道了一种采用圆柱形胶原-肝细胞凝胶的静态培养模型，用于对与三室中空纤维生物人工肝相关的条件进行大规模测试。通过将细胞包埋在胶原-肝细胞凝胶中来实现高密度肝细胞培养。通过活细胞染色、凝胶收缩和胰岛素利用来评估肝细胞活力。肝细胞特异性功能的指标包括白蛋白合成、尿素生成、利多卡因生物转化和胆酸盐结合。尽管在7天的孵育期内肝细胞活力保持稳定，但肝细胞功能并未得到一致的维持。白蛋白合成保持稳定，而代表性的P-450和结合活性随时间下降。这种静态培养系统将有助于开发利用圆柱形胶原-肝细胞凝胶的中空纤维生物人工肝。
