Widdicombe J H
Ciba Found Symp. 1984;109:109-20. doi: 10.1002/9780470720905.ch8.
Active ion transport across the surface epithelium of dog trachea results in fluid transport. The direction and amount of fluid transported may depend on a balance between active chloride secretion and active sodium absorption. In contrast, fluid movement across submucosal gland epithelium does not seem to depend on active salt transport. Instead, water may be drawn into the duct lumen by simple osmosis after the release of the osmotically active contents of secretory granules. Cell culture may provide a means of studying the secretory properties of the individual cell types of airway epithelia. We have produced primary monolayer cultures of cells from dog tracheal epithelium which retain the differentiated salt and water transport of the original tissue.
狗气管表面上皮的主动离子转运导致液体转运。液体转运的方向和量可能取决于主动氯离子分泌和主动钠离子吸收之间的平衡。相比之下，跨黏膜下腺上皮的液体移动似乎不依赖于主动盐转运。相反，分泌颗粒中具有渗透活性的内容物释放后，水可能通过简单的渗透作用被吸入导管腔。细胞培养可能提供一种研究气道上皮各细胞类型分泌特性的方法。我们已经从狗气管上皮细胞中制备了原代单层培养物，这些培养物保留了原始组织中分化的盐和水转运功能。
