Lam P, Stich H F
Can J Genet Cytol. 1978 Mar;20(1):35-40. doi: 10.1139/g78-006.
Exposure of cultured human fibroblasts to hyperthermia delayed the host-cell reactivation of UV-irradiated human adenovirus type 12 (AD12). The experimental design consisted of irradiating human AD12 with UV doses ranging from 180 to 1800 ergs/mm2, infecting human cell populations at 37 degrees C, exposing the infected cells for 7 h to 39.5 degrees C and 41.8 degrees C, returning them to 37 degrees C and estimating the frequency of cells with intranuclear viral inclusion bodies (IB) 41 and 89 h after hyperthermia treatment. Hyperthermia reduced the fractions of fibroblasts with viral IB in the 41 h samples. By 89 h the capacity to form IB in the treated cells was comparable to that in control cells. Hyperthermia of 39.5 and 41.8 degrees C for 7 h did not affect the replication of nonirradiated AD12. The pattern of host-cell reactivation of AD12 following hyperthermia was compared to that in DNA repair deficient xeroderma pigmentosum cell populations.
将培养的人成纤维细胞暴露于高温下会延迟紫外线照射的人腺病毒12型（AD12）的宿主细胞复活。实验设计包括用180至1800尔格/平方毫米的紫外线剂量照射人AD12，在37℃感染人细胞群体，将感染的细胞在39.5℃和41.8℃下暴露7小时，再将它们放回37℃，并在高温处理后41小时和89小时估计具有核内病毒包涵体（IB）的细胞频率。高温降低了41小时样本中具有病毒IB的成纤维细胞比例。到89小时时，处理过的细胞形成IB的能力与对照细胞相当。39.5℃和41.8℃的高温处理7小时不影响未照射的AD12的复制。将高温处理后AD12的宿主细胞复活模式与DNA修复缺陷的着色性干皮病细胞群体中的模式进行了比较。
