Moos M, Gallwitz D
EMBO J. 1983;2(5):757-61. doi: 10.1002/j.1460-2075.1983.tb01496.x.
From a human gene library we have isolated and sequenced a beta-actin-like pseudogene, H beta Ac-psi 2, which lacks intervening sequences and contains several mutations resulting in frame-shifts, stop codons and in a departure from the known beta-actin protein sequence. We have also extended our sequence work on the intronless human beta-actin-related pseudogene H beta Ac-psi 1 described previously and we find that both genes are processed genes ending in a poly(dA) tract and flanked by direct repeats. The gene H beta Ac-psi 2 is preceded by a 230-bp region in which the simple sequence 5'-GAAA-3' is repeated greater than 40 times. This satellite-like sequence is highly repetitive in the human genome.
我们从一个人类基因文库中分离并测序了一个β-肌动蛋白样假基因HβAc-psi 2,它没有内含子序列,包含多个导致移码、终止密码子以及与已知β-肌动蛋白蛋白质序列不同的突变。我们还扩展了之前描述的无内含子人类β-肌动蛋白相关假基因HβAc-psi 1的序列研究,发现这两个基因都是加工基因,以聚(dA)尾结束并两侧有正向重复序列。基因HβAc-psi 2之前有一个230 bp的区域,其中简单序列5'-GAAA-3'重复超过40次。这种卫星样序列在人类基因组中高度重复。