Tokunaga K, Yoda K, Sakiyama S
Nucleic Acids Res. 1985 May 10;13(9):3031-42. doi: 10.1093/nar/13.9.3031.
We have cloned a processed gene related to cytoplasmic gamma-actin from a phage library of mouse brain DNA. The gene lacks introns, carries a poly(dA) tract of 19-base pairs at the 3' end and is flanked by 13-base-pair direct repeats. Southern blotting experiments indicated that this segment is embedded in highly repetitive and strongly methylated sequences. Direct comparison of the flanking sequences with those of mouse repetitive DNAs revealed that this processed gene is integrated into a BAM5 family, which is one of the repetitive sequences of the mouse genome. In addition, it was shown that the direct repeats were created by a duplication of the corresponding 13-bp stretch of the BAM5 DNA as a consequence of the integration of this segment. These results strongly suggest that the processed gamma-actin gene has been conveyed to this locus through a poly(A)+ RNA intermediate by a transposon-like mechanism.
我们从小鼠脑DNA的噬菌体文库中克隆了一个与细胞质γ-肌动蛋白相关的加工基因。该基因没有内含子，在3'端有一个19个碱基对的聚（dA）序列，两侧是13个碱基对的直接重复序列。Southern印迹实验表明，该片段嵌入高度重复且高度甲基化的序列中。将侧翼序列与小鼠重复DNA的侧翼序列直接比较发现，这个加工基因整合到了BAM5家族中，BAM5家族是小鼠基因组的重复序列之一。此外，还表明直接重复序列是由于该片段的整合导致BAM5 DNA相应的13个碱基对片段重复而产生的。这些结果有力地表明，加工后的γ-肌动蛋白基因是通过类似转座子的机制通过聚（A）+ RNA中间体被转运到这个位点的。
