Engelhart M, Kristensen J K
J Invest Dermatol. 1983 Jan;80(1):12-5. doi: 10.1111/1523-1747.ep12530879.
A new method for noninvasive measurement of cutaneous blood flow is laser-Doppler flowmetry. The technique is based on the fact that laser light is back-scattered from the moving red blood cells, with Doppler-shifted frequencies; these impulses lead to photodetectors and are converted to flow signals. In this work we used a new system with a low noise level. Comparison was made between this technique and the atraumatic epicutaneous 133Xenon technique for measurement of cutaneous blood flow during reactive hyperemia and orthostatic pressure changes. The laser-Doppler flowmeter seems to measure blood flow in capillaries as well as in arteriovenous anastomoses, while the 133Xe method probably measures only capillary flow. A calibration of the laser-Doppler method against the 133Xe method would appear to be impossible in skin areas where arteriovenous anastomoses are present. The changes in blood flow during reactive hyperemia, orthostatic pressure changes, and venous stasis were found to be parallel as measured by the two methods in skin areas without shunt vessels. The laser-Doppler flowmeter would appear to be a useful supplement to the 133Xe washout method in cutaneous vascular physiology, but it is important to keep in mind that different parameters may be measured.
一种用于无创测量皮肤血流的新方法是激光多普勒血流仪。该技术基于这样一个事实，即激光被移动的红细胞反向散射，产生多普勒频移；这些脉冲被传送到光电探测器并转换为血流信号。在这项工作中，我们使用了一种具有低噪声水平的新系统。对该技术与无创表皮133氙技术在反应性充血和体位性压力变化期间测量皮肤血流的情况进行了比较。激光多普勒血流仪似乎能够测量毛细血管以及动静脉吻合处的血流，而133氙方法可能仅测量毛细血管血流。在存在动静脉吻合的皮肤区域，似乎不可能将激光多普勒方法与133氙方法进行校准。在没有分流血管的皮肤区域，通过这两种方法测量发现，反应性充血、体位性压力变化和静脉淤滞期间的血流变化是平行的。在皮肤血管生理学中，激光多普勒血流仪似乎是对133氙清除法的一种有用补充，但重要的是要记住，可能测量的是不同参数。
