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[Staining the Nissl bodies in 4--10 microns thick Araldite sections with an area of about 2x2 cm (author's transl)].

作者信息

Braak E

出版信息

Microsc Acta. 1976 Sep;78(4):289-91.

PMID:65719
Abstract

For light microscopy 4--10 microns thick sections with an area of 2x2 cm o Araldite-embedded tissue (human autopsy brain) are stained in 1% aqueous methylene blue at 65 degrees C for 4--12 hours and are differentiated in 98% ethanol for some seconds or very few minutes. The Nissl bodies, nuclei and nucleoli are stained dark blue, the neuropil is nearly colourless.

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