del Cerro M, Cogen J, del Cerro C
Microsc Acta. 1980 May;83(2):117-21.
Stevenel's Blue is a reliable, rapid, and clean, one-step polychromatic stain for 1 micron thick epoxy sections. The staining solution, originally used by L. Stevenel (1918) to stain human parasites, is made by adding diluted potassium permanganate (2%) to an aqueous solution of the methylene blue (1.3%) and redissolving the precipitate thoroughly, by boiling in water bath and filtering. Staining is carried out in a Coplin jar at 60 degrees C for approximately 10 minutes for tissues embedded in Epon 812 or Poly 812, or 20 minutes for tissues embedded in Spurr's medium. The sections are rinsed, air dried, and mouted in Permount. The staining solution is very stable, and does not tend to form precipitates on the tissue. The stain brings excellent histological differentiation to nuclear, cytoplasmic, and extracellular components. Incorporation of the stain by elements within each tissue varies from intense to light with a subtle gradation of intermediate shades of purple and blue tones. For most cell structures the density of the stain parallels the electron density of that structure as seen under the electron microscope. For example, nucleoli and heterochromatin stain in dark purple while euterochromatin appear in a light blue shade. In all cases, the embedding media remains unstained. The bond between Stevenel's Blue and the tissues is stable, remaining unaltered by the mounting medium. It is also resistant to time-fading.
史蒂文内尔蓝染液是一种可靠、快速且干净的一步多色染色剂，用于1微米厚的环氧树脂切片。该染色液最初由L. 史蒂文内尔（1918年）用于染色人体寄生虫，其制备方法是将稀释的高锰酸钾（2%）加入亚甲蓝水溶液（1.3%）中，通过在水浴中煮沸并充分溶解沉淀物，然后过滤。对于包埋在Epon 812或Poly 812中的组织，在60摄氏度的科普林缸中染色约10分钟；对于包埋在斯珀树脂中的组织，则染色20分钟。切片冲洗后，空气干燥，并用Permount封固。该染色液非常稳定，不易在组织上形成沉淀。这种染色能使细胞核、细胞质和细胞外成分呈现出极佳的组织学分化。每个组织中的元素对染色液的摄取程度从深到浅不等，带有紫色和蓝色调的微妙中间渐变。对于大多数细胞结构，染色的密度与在电子显微镜下观察到的该结构的电子密度相似。例如，核仁和异染色质染成深紫色，而常染色质则呈现浅蓝色。在所有情况下，包埋介质均不着色。史蒂文内尔蓝染液与组织之间的结合稳定，不会因封固介质而改变。它也具有抗褪色性。
