Greenberger J S, Hoffman N, Lieberman M, Botnick L E, Sakakeeny M A, Eckner R J
J Natl Cancer Inst. 1983 Feb;70(2):323-31.
Continuous mouse bone marrow cultures were infected with Friend murine leukemia virus. Production of nonadherent (NA) and adherent cells, granulocyte-macrophage colony-forming unit(s) of progenitor cells (GM-CFUc), pluripotential hematopoietic stem cells (CFUs), the self-renewal potential (Rs) of CFUs, and generation of factor-dependent (FD) multipotential and committed permanent stem cell cloned lines were measured. Uninfected marrow cultures from C57BL/6J, C57BL/6JUt, B6.S, C3H/HeJ, (C57BL/6J x DBA/2J)F1, CD- 1 Swiss, or N:NIH(S) mice generated NA cells, GM-CFUc, and CFUs for 20-41 weeks; cultures infected with Rauscher or other helper viruses generated them for 35-45 weeks. GM-CFUc and CFUs production in SFFV-positive cultures persisted for over 65 weeks and exceeded control levels by twentyfold to fiftyfold. The Rs of CFUs in SFFV-positive cultures was not detectably increased above control cultures. Multipotential (erythroid-neutrophil-mast cell-basophil-eosinophil) permanent FD cell clones were derived from control and SFFV-positive cultures. Thus SFFV amplifies the stem cell pool in vitro without detectably increasing the Rs capacity of CFUs.
连续的小鼠骨髓培养物用Friend小鼠白血病病毒感染。测量非贴壁（NA）细胞和贴壁细胞的产生、祖细胞的粒细胞-巨噬细胞集落形成单位（GM-CFUc）、多能造血干细胞（CFU）、CFU的自我更新潜能（Rs）以及因子依赖性（FD）多能和定向永久性干细胞克隆系的生成。来自C57BL/6J、C57BL/6JUt、B6.S、C3H/HeJ、（C57BL/6J×DBA/2J）F1、CD-1瑞士小鼠或N:NIH（S）小鼠的未感染骨髓培养物产生NA细胞、GM-CFUc和CFU达20 - 41周；用Rauscher或其他辅助病毒感染的培养物产生这些细胞达35 - 45周。SFFV阳性培养物中GM-CFUc和CFU的产生持续超过65周，且超过对照水平二十倍至五十倍。SFFV阳性培养物中CFU的Rs在对照培养物之上未检测到明显增加。多能（红系-中性粒细胞-肥大细胞-嗜碱性粒细胞-嗜酸性粒细胞）永久性FD细胞克隆源自对照和SFFV阳性培养物。因此，SFFV在体外扩增干细胞池，而未检测到CFU的Rs能力明显增加。
