Greenberger J S, Eckner R J, Sakakeeny M A, Cohen G I
Leuk Res. 1983;7(5):621-36. doi: 10.1016/0145-2126(83)90133-9.
Replication of the Friend spleen focus forming virus (SFFV) in C3H/HeJ or C57BL/6J mouse continuous bone marrow cultures is associated with an increased cumulative production of pluripotential/hematopoietic stem cells (CFUs), granulocyte-macrophage progenitor cells (GM-CFUc), and total granulocytes, compared to uninfected or helper virus infected control cultures. The site(s) of action of the virus are not known. To determine whether viral effect(s) occurred in adherent stromal and/or non-adherent hematopoietic stem cells, purified cell populations, comprised exclusively of cells from each compartment, were separated from C57BL/6J (Fv-1bb) or C3H/HeJ (Fv-1nn) marrow cultures and were left uninfected or were infected with host range replication restricted B-tropic or N-tropic helper pseudotype viruses of SFFV respectively. Cell populations were then mixed to establish continuous hematopoiesis in allogeneic or syngeneic combinations. Virus host range restriction between compartments was maintained in allogeneic reconstituted cultures which showed active hematopoiesis for 16-17 weeks and no significant differences between: (1) virus infected and uninfected groups or (2) groups comprised of stromal cells from one or the other strain. Thus, these data indicate that the prolongation of hematopoiesis in undisturbed long-term marrow cultures by SFFV occurs through an interaction of adherent hematopoietic stem cells with the marrow stroma.
与未感染或感染辅助病毒的对照培养物相比，Friend脾集落形成病毒（SFFV）在C3H/HeJ或C57BL/6J小鼠连续骨髓培养物中的复制与多能/造血干细胞（CFU）、粒细胞-巨噬细胞祖细胞（GM-CFUc）和总粒细胞的累积产量增加有关。病毒的作用位点尚不清楚。为了确定病毒效应是否发生在贴壁基质细胞和/或非贴壁造血干细胞中，从C57BL/6J（Fv-1bb）或C3H/HeJ（Fv-1nn）骨髓培养物中分离出仅由每个区室的细胞组成的纯化细胞群体，使其保持未感染状态或分别感染宿主范围复制受限的SFFV B嗜性或N嗜性辅助假型病毒。然后将细胞群体混合，以同种异体或同基因组合建立连续造血。在显示活跃造血16-17周的同种异体重建培养物中，区室之间的病毒宿主范围限制得以维持，并且在以下两组之间没有显著差异：（1）病毒感染组和未感染组，或（2）由一种或另一种品系的基质细胞组成的组。因此，这些数据表明，SFFV在未受干扰的长期骨髓培养物中延长造血作用是通过贴壁造血干细胞与骨髓基质的相互作用实现的。
