通过蛋白质二维微量凝胶电泳对淋巴细胞进行表征
Characterization of lymphoid cells by two-dimensional mini gel electrophoresis of proteins.
作者信息
Hirsch F W, Bröckl C, Bross K J, Dölken G
出版信息
J Cancer Res Clin Oncol. 1983;105(2):166-72. doi: 10.1007/BF00406928.
Cytosol proteins from normal lymphocytes, leukemic lymphocytes, and different cultured lymphoid cell lines were separated by two-dimensional mini gel electrophoresis. By staining with Coomassie blue, specific protein patterns were obtained. Very similar gel maps were produced by the cytosol proteins of chronic lymphocytic leukemia cells, hairy cells, and of in vitro grown B cells. Protein 36/6.2 (molecular weight/isoelectric point), consistently present in these cells, could not be demonstrated in normal lymphocytes. For the comparison of control Raji cells--an Epstein-Barr-Virus (EBV)-DNA carrying Burkitt's lymphoma cell line--with Raji cells induced for early antigen (EA) production, 35S-methionine-labelled total cell lysates were analyzed. In the induced cells, an additional protein (100/5.5) was found; this might be one of the immunologically defined EBV-associated antigens. These results demonstrate that two-dimensional mini gel electrophoresis can be useful for the characterization of leukemic cells in addition to the morphological, cytochemical, and surface marker analyses.
通过二维微量凝胶电泳分离正常淋巴细胞、白血病淋巴细胞及不同培养的淋巴样细胞系的胞质溶胶蛋白。用考马斯亮蓝染色后，获得了特异性蛋白质图谱。慢性淋巴细胞白血病细胞、毛细胞及体外培养的B细胞的胞质溶胶蛋白产生了非常相似的凝胶图谱。在这些细胞中始终存在的蛋白质36/6.2（分子量/等电点），在正常淋巴细胞中未检测到。为了比较对照Raji细胞（一种携带爱泼斯坦-巴尔病毒（EBV）DNA的伯基特淋巴瘤细胞系）与诱导产生早期抗原（EA）的Raji细胞，分析了用35S-甲硫氨酸标记的全细胞裂解物。在诱导细胞中发现了一种额外的蛋白质（100/5.5）；这可能是免疫定义的EBV相关抗原之一。这些结果表明，除形态学、细胞化学和表面标志物分析外，二维微量凝胶电泳可用于白血病细胞的特征鉴定。
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