Braun M P, Martin P J, Ledbetter J A, Hansen J A
Blood. 1983 Apr;61(4):718-25.
We have investigated the expression of the common acute lymphoblastic leukemia antigen (cALLA) by nonlymphoid cells, using a new murine monoclonal antibody (designated 24.1), specific for cALLA. This antibody completely blocks the binding of monoclonal anti-cALLA antibody J-5. Furthermore, antibody 24.1 binds to cALLA with greater affinity and induces a greater degree of antigenic modulation than antibody J-5. With antibody 24.1, we have demonstrated that normal cultured marrow and skin fibroblasts and mature granulocytes express cALLA. By immune precipitation and polyacrylamide gel electrophoresis, cALLA from fibroblasts has a slightly lower molecular weight and cALLA from granulocytes a slightly higher molecular weight than cALLA from cultured human leukemic cell lines. Quantitative studies indicate, however, that cALLA expression is approximately 3-fold and 20-fold lower, respectively, on normal granulocytes and skin fibroblasts than on NALM-6 cells. cALLA may be expressed by cells with widespread distribution in multiple organ systems. These findings emphasize that differentiation markers that appear to be tumor or tissue specific may be found on cells of diverse origin.
我们使用一种针对普通急性淋巴细胞白血病抗原（cALLA）的新型鼠单克隆抗体（命名为24.1），研究了非淋巴细胞对该抗原的表达情况。这种抗体完全阻断了单克隆抗cALLA抗体J-5的结合。此外，与抗体J-5相比，抗体24.1与cALLA的结合亲和力更高，且诱导的抗原调变程度更大。利用抗体24.1，我们证明了正常培养的骨髓和皮肤成纤维细胞以及成熟粒细胞表达cALLA。通过免疫沉淀和聚丙烯酰胺凝胶电泳分析，成纤维细胞中的cALLA分子量略低于培养的人白血病细胞系中的cALLA，而粒细胞中的cALLA分子量略高于培养的人白血病细胞系中的cALLA。然而，定量研究表明，正常粒细胞和皮肤成纤维细胞上的cALLA表达分别比NALM-6细胞低约3倍和20倍。cALLA可能由在多个器官系统中广泛分布的细胞表达。这些发现强调，看似肿瘤或组织特异性的分化标志物可能存在于多种来源的细胞上。
