Woods D E, Fitschen W
Cell Differ. 1978 Apr;7(1-2):103-14. doi: 10.1016/0045-6039(78)90011-8.
The extent of protein, RNA and DNA synthesis in early cleavage stages of the sea urchin embryo (Parechinus angulosus) was determined. A histone mRNA specific cDNA was used in hybridization experiments to investigate the cytoplasmic localization of maternal histone mRNA in the unfertilized sea urchin egg and first cleavage stage embryo. In the unfertilized egg histone mRNA was localized exclusively in ribonucleoprotein particles with none in ribosomes or polyribosomes. This distribution changed after fertilization, in particular, coupled with the first cleavage telophase there was a significant transfer of histone mRNA from the ribonucleoprotein fraction to the polyribosomes. The results indicate mRNA specific translational control mechanisms.
测定了海胆胚胎（角海胆）早期卵裂阶段蛋白质、RNA和DNA的合成程度。在杂交实验中使用一种组蛋白mRNA特异性cDNA，以研究母体组蛋白mRNA在未受精海胆卵和第一次卵裂阶段胚胎中的细胞质定位。在未受精卵中，组蛋白mRNA仅定位在核糖核蛋白颗粒中，核糖体或多核糖体中没有。受精后这种分布发生了变化，特别是在第一次卵裂末期，组蛋白mRNA从核糖核蛋白组分大量转移到多核糖体中。结果表明存在mRNA特异性翻译控制机制。
