Finan P J, Wight D G, Lennox E S, Sacks S H, Bleehen N M
J Natl Cancer Inst. 1983 Apr;70(4):679-85.
Variation in human blood group isoantigen expression on normal and malignant gastric epithelium was demonstrated with monoclonal antibodies to blood groups A and B in an indirect immunoperoxidase technique. The expected isoantigen expression was demonstrated on endoscopic biopsy specimens of normal gastric mucosa from 11 patients. Of 17 patients with gastric carcinoma (blood group A, 15; blood group AB, 2), complete loss of isoantigen expression was noted in 6 (35%). In these 6 patients, blood group isoantigen remained both in the adjacent uninvolved mucosa and at the margin of resection. The loss of isoantigen did not appear to be related to the degree of differentiation within the tumor, to the secretor status of the patient, or to the blood subgroup. Lymph node metastases reflected the isoantigen status of the primary tumor, being positive in 5 of 6 expression in all 17 patients or in an additional 15 patients studied with blood group O. These findings were discussed in the light of previously reported work on the localization of blood group isoantigens on malignant and nonmalignant gastric epithelium with the use of conventional antisera and a variety of immunohistologic techniques.
采用间接免疫过氧化物酶技术，利用抗A、B血型的单克隆抗体，证实了正常和恶性胃上皮细胞上人类血型同种抗原表达的差异。在11例患者正常胃黏膜的内镜活检标本上证实了预期的同种抗原表达。在17例胃癌患者中（A型血15例，AB型血2例），6例（35%）出现同种抗原表达完全缺失。在这6例患者中，血型同种抗原在相邻未受累黏膜及切除边缘均存在。同种抗原的缺失似乎与肿瘤内的分化程度、患者的分泌状态或血型亚型无关。淋巴结转移反映了原发肿瘤的同种抗原状态，在6例表达缺失的患者中有5例出现转移，在所有17例患者或另外15例O型血患者的研究中均是如此。根据先前报道的使用传统抗血清和各种免疫组织学技术对恶性和非恶性胃上皮细胞血型同种抗原定位的研究结果，对这些发现进行了讨论。
