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一种激活剂和一种从牛肾上腺皮质细胞质中分离出的抑制剂对胆固醇侧链裂解动力学的调节作用。

Modulation of the kinetics of cholesterol side-chain cleavage by an activator and by an inhibitor isolated from the cytosol of the cortex of bovine adrenals.

作者信息

Warne P A, Greenfield N J, Lieberman S

出版信息

Proc Natl Acad Sci U S A. 1983 Apr;80(7):1877-81. doi: 10.1073/pnas.80.7.1877.

DOI:10.1073/pnas.80.7.1877
PMID:6572949
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC393713/
Abstract

Two modulators of sterol side-chain cleavage activity have been detected in the cytosol from the cortex of bovine adrenals. One is an inhibitor of side-chain cleavage which increases the Km of a purified and reconstituted mitochondrial side-chain cleavage system for both cholesterol and cholesterol sulfate. It also lowers the Vmax of cleavage when cholesterol sulfate is the substrate. The other modulator is a low molecular weight protein which in the reconstituted system increases the Vmax of cleavage for both substrates but does not affect the Km of either. Side-chain cleavage activity was also found in bovine adrenocortical cytosol and this appears to differ from that present in mitochondria.

摘要

在牛肾上腺皮质的胞质溶胶中已检测到两种甾醇侧链裂解活性调节剂。一种是侧链裂解抑制剂，它会增加纯化并重构的线粒体侧链裂解系统对胆固醇和硫酸胆固醇的米氏常数（Km）。当以硫酸胆固醇为底物时，它还会降低裂解反应的最大反应速度（Vmax）。另一种调节剂是一种低分子量蛋白质，在重构系统中，它会增加两种底物的裂解反应Vmax，但不影响任何一种底物的Km。在牛肾上腺皮质胞质溶胶中也发现了侧链裂解活性，这似乎与线粒体中的活性不同。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d3/393713/8755baef658a/pnas00633-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d3/393713/8755baef658a/pnas00633-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d3/393713/8755baef658a/pnas00633-0102-a.jpg

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EVIDENCE THAT STEROID SULFATES SERVE AS BIOSYNTHETIC INTERMEDIATES. IV. CONVERSION OF CHOLESTEROL SULFATE IN VIVO TO URINARY C-19 AND C-21 STEROIDAL SULFATES.硫酸类固醇作为生物合成中间体的证据。IV. 体内胆固醇硫酸酯向尿中C-19和C-21甾体硫酸酯的转化
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Proc Natl Acad Sci U S A. 1983 Apr;80(7):1882-6. doi: 10.1073/pnas.80.7.1882.
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Phospholipid vesicle-reconstituted cytochrome P-450SCC. Mutually facilitated binding of cholesterol and adrenodoxin.
J Biol Chem. 1980 Jan 10;255(1):138-43.
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The lipoidal derivatives of steroids as biosynthetic intermediates.作为生物合成中间体的类固醇类脂衍生物。
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Mitochondrial cytochrome P-450scc. Mechanism of electron transport by adrenodoxin.线粒体细胞色素P-450侧链裂解酶。肾上腺皮质铁氧化还原蛋白的电子传递机制。
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Induction of synthesis of cholesterol side chain cleavage cytochrome P-450 by adrenocorticotropin in cultured bovine adrenocortical cells.促肾上腺皮质激素在培养的牛肾上腺皮质细胞中诱导胆固醇侧链裂解细胞色素P-450的合成。
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