内吞作用抑制剂会扰乱兔中性粒细胞及其他细胞中的磷脂代谢。
Inhibitors of endocytosis perturb phospholipid metabolism in rabbit neutrophils and other cells.
作者信息
Mato J M, Pencev D, Vasanthakumar G, Schiffmann E, Pastan I
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(7):1929-32. doi: 10.1073/pnas.80.7.1929.
Dansylcadaverine, amantadine, and rimantadine, which have been shown to inhibit the endocytosis of alpha 2-macroglobulin, epidermal growth factor, and vesicular stomatitis virus [Schlegel, R., Dickson, R. B., Willingham, M. C. & Pastan, I. (1982) Proc. Natl. Acad. Sci. USA 79, 2291-2295], were found to decrease phosphatidylcholine synthesis, chemotaxis, and internalization of a formylated peptide but to stimulate the incorporation of inositol into phosphatidylinositol in rabbit neutrophils. Dansylcadaverine decreased phosphatidylcholine synthesis by both the CDP-choline and transmethylation pathways and also inhibited the synthesis of phosphatidylethanolamine by the CDP-ethanolamine pathway. Dansylcadaverine had no effect on the phosphocholine, CDP-choline, or S-adenosyl-L-homocysteine pools but increased 2-fold the S-adenosyl-L-methionine pool. These results suggest that dansylcadaverine in some manner inhibited the condensation of CDP-choline with diacylglycerol to form phosphatidylcholine. Dansylcadaverine also inhibited phosphatidylcholine synthesis in human neutrophils, human fibroblasts, chicken embryo fibroblasts, rat hepatocytes, osteosarcoma cells, and neuroblastoma cells. It did not stimulate phosphatidylinositol synthesis in chicken embryo fibroblasts.
丹磺酰尸胺、金刚烷胺和甲基金刚烷胺已被证明可抑制α2-巨球蛋白、表皮生长因子和水泡性口炎病毒的内吞作用[施莱格尔，R.，迪克森，R. B.，韦林厄姆，M. C. & 帕斯坦，I.（1982年）《美国国家科学院院刊》79，2291 - 2295]，研究发现它们可降低兔中性粒细胞中磷脂酰胆碱的合成、趋化性以及甲酰化肽的内化作用，但能刺激肌醇掺入磷脂酰肌醇。丹磺酰尸胺通过CDP - 胆碱和转甲基化途径降低磷脂酰胆碱的合成，还通过CDP - 乙醇胺途径抑制磷脂酰乙醇胺的合成。丹磺酰尸胺对磷酸胆碱、CDP - 胆碱或S - 腺苷 - L - 同型半胱氨酸池无影响，但使S - 腺苷 - L - 甲硫氨酸池增加了2倍。这些结果表明，丹磺酰尸胺以某种方式抑制了CDP - 胆碱与二酰甘油缩合形成磷脂酰胆碱。丹磺酰尸胺还抑制人中性粒细胞、人成纤维细胞、鸡胚成纤维细胞、大鼠肝细胞、骨肉瘤细胞和神经母细胞瘤细胞中磷脂酰胆碱的合成。它对鸡胚成纤维细胞中的磷脂酰肌醇合成无刺激作用。
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