Leprohon C E, Blusztajn J K, Wurtman R J
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2063-6. doi: 10.1073/pnas.80.7.2063.
Rat brain synaptosomes contain enzymes, phosphatidylethanolamine N-methyltransferase(s) (EC 2.1.1.17), that catalyze the methylation of endogenous phosphatidylethanolamine to form its mono-, di-, and trimethyl (i.e., phosphatidylcholine) derivatives. We observe that the activity of these enzymes is enhanced when synaptosomes are incubated with catecholamines: 0.1 mM dopamine increases incorporation of [3H]methyl groups into monomethylphosphatidylethanolamine, dimethylphosphatidylethanolamine, and phosphatidylcholine by factors of 1.7, 1.3, and 2.1, respectively, and 0.1 mM norepinephrine increases [3H]methyl incorporation into monomethylphosphatidylethanolamine and dimethylphosphatidylethanolamine by factors of 1.6 and 2.1, respectively. Stimulation by dopamine, which is observed at concentrations as low as 1 microM, is blocked by haloperidol.
大鼠脑突触体含有磷脂酰乙醇胺N-甲基转移酶（EC 2.1.1.17）等酶，这些酶催化内源性磷脂酰乙醇胺甲基化，形成其一甲基、二甲基和三甲基（即磷脂酰胆碱）衍生物。我们观察到，当突触体与儿茶酚胺一起孵育时，这些酶的活性会增强：0.1 mM多巴胺使[3H]甲基掺入单甲基磷脂酰乙醇胺、二甲基磷脂酰乙醇胺和磷脂酰胆碱的量分别增加1.7倍、1.3倍和2.1倍，0.1 mM去甲肾上腺素使[3H]甲基掺入单甲基磷脂酰乙醇胺和二甲基磷脂酰乙醇胺的量分别增加1.6倍和2.1倍。低至1 microM的多巴胺浓度即可观察到刺激作用，该作用可被氟哌啶醇阻断。
