多巴胺对大鼠脑神经元中磷脂酰胆碱（卵磷脂）生物合成的刺激作用。
Dopamine stimulation of phosphatidylcholine (lecithin) biosynthesis in rat brain neurons.
作者信息
Leprohon C E, Blusztajn J K, Wurtman R J
出版信息
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2063-6. doi: 10.1073/pnas.80.7.2063.
Abstract
Rat brain synaptosomes contain enzymes, phosphatidylethanolamine N-methyltransferase(s) (EC 2.1.1.17), that catalyze the methylation of endogenous phosphatidylethanolamine to form its mono-, di-, and trimethyl (i.e., phosphatidylcholine) derivatives. We observe that the activity of these enzymes is enhanced when synaptosomes are incubated with catecholamines: 0.1 mM dopamine increases incorporation of [3H]methyl groups into monomethylphosphatidylethanolamine, dimethylphosphatidylethanolamine, and phosphatidylcholine by factors of 1.7, 1.3, and 2.1, respectively, and 0.1 mM norepinephrine increases [3H]methyl incorporation into monomethylphosphatidylethanolamine and dimethylphosphatidylethanolamine by factors of 1.6 and 2.1, respectively. Stimulation by dopamine, which is observed at concentrations as low as 1 microM, is blocked by haloperidol.
摘要
大鼠脑突触体含有磷脂酰乙醇胺N-甲基转移酶（EC 2.1.1.17）等酶，这些酶催化内源性磷脂酰乙醇胺甲基化，形成其一甲基、二甲基和三甲基（即磷脂酰胆碱）衍生物。我们观察到，当突触体与儿茶酚胺一起孵育时，这些酶的活性会增强：0.1 mM多巴胺使[3H]甲基掺入单甲基磷脂酰乙醇胺、二甲基磷脂酰乙醇胺和磷脂酰胆碱的量分别增加1.7倍、1.3倍和2.1倍，0.1 mM去甲肾上腺素使[3H]甲基掺入单甲基磷脂酰乙醇胺和二甲基磷脂酰乙醇胺的量分别增加1.6倍和2.1倍。低至1 microM的多巴胺浓度即可观察到刺激作用，该作用可被氟哌啶醇阻断。
相似文献
1
Dopamine stimulation of phosphatidylcholine (lecithin) biosynthesis in rat brain neurons.多巴胺对大鼠脑神经元中磷脂酰胆碱（卵磷脂）生物合成的刺激作用。
Proc Natl Acad Sci U S A. 1983 Apr;80(7):2063-6. doi: 10.1073/pnas.80.7.2063.
2
Phosphatidylcholine produced in rat synaptosomes by N-methylation is enriched in polyunsaturated fatty acids.大鼠突触小体中通过N-甲基化产生的磷脂酰胆碱富含多不饱和脂肪酸。
Proc Natl Acad Sci U S A. 1985 Jul;82(14):4828-31. doi: 10.1073/pnas.82.14.4828.
3
Synthesis of lecithin (phosphatidylcholine) from phosphatidylethanolamine in bovine brain.牛脑中由磷脂酰乙醇胺合成卵磷脂（磷脂酰胆碱）
Brain Res. 1979 Dec 28;179(2):319-27. doi: 10.1016/0006-8993(79)90447-5.
4
Choline biosynthesis by a preparation enriched in synaptosomes from rat brain.通过富含大鼠脑突触体的制剂进行胆碱生物合成。
Nature. 1981 Apr 2;290(5805):417-8. doi: 10.1038/290417a0.
5
6
Regulation of phosphatidylcholine biosynthesis by the methylation pathway in rat pituitary gland.
Horm Metab Res. 1984 Aug;16(8):431-4. doi: 10.1055/s-2007-1014809.
7
Estradiol activates methylating enzyme(s) involved in the conversion of phosphatidylethanolamine to phosphatidylcholine in rat pituitary membranes.雌二醇激活大鼠垂体膜中参与磷脂酰乙醇胺转化为磷脂酰胆碱的甲基化酶。
Endocrinology. 1986 Dec;119(6):2611-22. doi: 10.1210/endo-119-6-2611.
8
Rat and human mammary tissue can synthesize choline moiety via the methylation of phosphatidylethanolamine.大鼠和人类乳腺组织可通过磷脂酰乙醇胺的甲基化作用合成胆碱部分。
Biochem J. 1988 Dec 15;256(3):821-8. doi: 10.1042/bj2560821.
9
Sarcolemmal phosphatidylethanolamine N-methylation in diabetic cardiomyopathy.糖尿病性心肌病中的肌膜磷脂酰乙醇胺N-甲基化
Circ Res. 1984 Oct;55(4):504-12. doi: 10.1161/01.res.55.4.504.
10
Expression of phosphatidylethanolamine N-methyltransferase-2 in McArdle-RH7777 hepatoma cells inhibits the CDP-choline pathway for phosphatidylcholine biosynthesis via decreased gene expression of CTP:phosphocholine cytidylyltransferase.磷脂酰乙醇胺N-甲基转移酶-2在McArdle-RH7777肝癌细胞中的表达通过降低CTP：磷酸胆碱胞苷转移酶的基因表达来抑制磷脂酰胆碱生物合成的CDP-胆碱途径。
Biochem J. 1995 Dec 15;312 ( Pt 3)(Pt 3):939-45. doi: 10.1042/bj3120939.
引用本文的文献
1
Transdiagnostic reduction in cortical choline-containing compounds in anxiety disorders: a H-magnetic resonance spectroscopy meta-analysis.焦虑症中皮质含胆碱化合物的跨诊断减少：一项氢磁共振波谱荟萃分析
Mol Psychiatry. 2025 Sep 5. doi: 10.1038/s41380-025-03206-7.
2
Serum Phospholipid Profile Changes in Gaucher Disease and Parkinson's Disease.戈谢病和帕金森病患者血清磷脂谱的变化
Int J Mol Sci. 2022 Sep 8;23(18):10387. doi: 10.3390/ijms231810387.
3
Increased levels of methylated intermediates of phosphatidylcholine lead to enhanced phospholipase D activity.磷脂酰胆碱甲基化中间体水平升高会导致磷脂酶D活性增强。
Neurochem Res. 1998 Aug;23(8):1099-105. doi: 10.1023/a:1020716304520.
4
Entry of polyunsaturated fatty acids into the brain: evidence that high-density lipoprotein-induced methylation of phosphatidylethanolamine and phospholipase A2 are involved.多不饱和脂肪酸进入大脑：有证据表明高密度脂蛋白诱导的磷脂酰乙醇胺甲基化和磷脂酶A2参与其中。
Biochem J. 1996 Jun 15;316 ( Pt 3)(Pt 3):805-11. doi: 10.1042/bj3160805.
5
The substrate specificity of brain microsomal phospholipase D.脑微粒体磷脂酶D的底物特异性
Biochem J. 1993 Nov 1;295 ( Pt 3)(Pt 3):793-8. doi: 10.1042/bj2950793.
6
Insulin stimulation of phospholipid methylation in isolated rat adipocyte plasma membranes.胰岛素对分离的大鼠脂肪细胞质膜中磷脂甲基化的刺激作用。
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1089-92. doi: 10.1073/pnas.81.4.1089.
7
Developmental changes in the activity of phosphatidylethanolamine N-methyltransferases in rat brain.大鼠脑中磷脂酰乙醇胺N-甲基转移酶活性的发育变化
Biochem J. 1985 Dec 1;232(2):505-11. doi: 10.1042/bj2320505.
8
Phosphatidylcholine produced in rat synaptosomes by N-methylation is enriched in polyunsaturated fatty acids.大鼠突触小体中通过N-甲基化产生的磷脂酰胆碱富含多不饱和脂肪酸。
Proc Natl Acad Sci U S A. 1985 Jul;82(14):4828-31. doi: 10.1073/pnas.82.14.4828.
9
Day-night cycle of lipidic composition in rat cerebral cortex.
Neurochem Res. 1987 Apr;12(4):315-21. doi: 10.1007/BF00993238.
本文引用的文献
1
Identification and properties of methyltransferases that synthesize phosphatidylcholine in rat brain synaptosomes.大鼠脑突触体中合成磷脂酰胆碱的甲基转移酶的鉴定及特性
J Neurochem. 1980 Jun;34(6):1491-8. doi: 10.1111/j.1471-4159.1980.tb11229.x.
2
The use of 1,6-diphenylhexatriene to detect lipids on thin-layer chromatograms.使用1,6 - 二苯基己三烯检测薄层色谱上的脂质。
Anal Biochem. 1980 Jan 1;101(1):75-7. doi: 10.1016/0003-2697(80)90042-1.
3
A rapid method for preparing synaptosomes: comparison, with alternative procedures.一种制备突触体的快速方法：与其他方法的比较。
Brain Res. 1981 Dec 7;226(1-2):107-18. doi: 10.1016/0006-8993(81)91086-6.
4
Choline biosynthesis by a preparation enriched in synaptosomes from rat brain.通过富含大鼠脑突触体的制剂进行胆碱生物合成。
Nature. 1981 Apr 2;290(5805):417-8. doi: 10.1038/290417a0.
5
Phospholipid methyltransferase asymmetry in synaptosomal membranes.突触体膜中磷脂甲基转移酶的不对称性
Neurochem Res. 1980 Sep;5(9):983-91. doi: 10.1007/BF00966137.
6
Properties of particulate and detergent-solubilized phospholipid N-methyltransferase activity from calf brain.来自小牛脑的颗粒状和去污剂增溶的磷脂N-甲基转移酶活性的特性
J Neurochem. 1982 May;38(5):1404-12. doi: 10.1111/j.1471-4159.1982.tb07919.x.
7
Phospholipid methylation: a biochemical signal modulating lymphocyte mitogenesis.磷脂甲基化：一种调节淋巴细胞有丝分裂的生化信号。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):862-5. doi: 10.1073/pnas.77.2.862.
8
Evidence for a coupling between dopaminergic receptors and phospholipid methylation in mouse B lymphocytes.小鼠B淋巴细胞中多巴胺能受体与磷脂甲基化之间偶联的证据。
Life Sci. 1981 Dec 28;29(26):2737-49. doi: 10.1016/0024-3205(81)90533-6.
9
Stimulation by vasopressin and angiotensin of phospholipid methyltransferase in isolated rat hepatocytes.血管加压素和血管紧张素对离体大鼠肝细胞中磷脂甲基转移酶的刺激作用。
FEBS Lett. 1981 Nov 30;135(1):111-4. doi: 10.1016/0014-5793(81)80955-6.
10
Forskolin: a unique diterpene activator of cyclic AMP-generating systems.福司可林：一种独特的环磷酸腺苷生成系统二萜激活剂。
J Cyclic Nucleotide Res. 1981;7(4):201-24.
Zotero 插件