Patel D J, Ikuta S, Kozlowski S, Itakura K
Proc Natl Acad Sci U S A. 1983 Apr;80(8):2184-8. doi: 10.1073/pnas.80.8.2184.
The kinetics for hydrogen exchange at individual base pairs in self-complementary deoxydodecanucleotide duplexes have been estimated from NMR saturation recovery measurements on the resolved imino protons as a function of temperature. The imino protons of dA . dT base pairs in the center of the fully alternating d(C-G-C-G-T-A-T-A-C-G-C-G) duplex exchange a factor of 2- to 3-fold faster than the corresponding protons at the same positions in the partially alternating d(C-G-C-G-A-A-T-T-C-G-C-G) duplex. These exchange parameters are a direct measure of the rate constants for transient opening of individual dA . dT base pairs in the dodecanucleotide duplexes and demonstrate faster opening kinetics for the "TATA" box region compared to the related "AATT" segment.
通过对已解析的亚氨基质子进行核磁共振饱和恢复测量,并将其作为温度的函数,估算了自互补脱氧十二核苷酸双链体中各个碱基对处氢交换的动力学。在完全交替的d(C-G-C-G-T-A-T-A-C-G-C-G)双链体中心,dA·dT碱基对的亚氨基质子交换速度比部分交替的d(C-G-C-G-A-A-T-T-C-G-C-G)双链体中相同位置的相应质子快2至3倍。这些交换参数是十二核苷酸双链体中单个dA·dT碱基对瞬时打开速率常数的直接度量,表明与相关的“AATT”片段相比,“TATA”框区域的打开动力学更快。