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抗生素与DNA的相互作用：溶液中鱼精蛋白-d（C-G-C-G-A-A-T-T-C-G-C-G）复合物中的分子间核Overhauser效应

Antibiotic-DNA interactions: intermolecular nuclear Overhauser effects in the netropsin-d(C-G-C-G-A-A-T-T-C-G-C-G) complex in solution.

作者信息

Patel D J

出版信息

Proc Natl Acad Sci U S A. 1982 Nov;79(21):6424-8. doi: 10.1073/pnas.79.21.6424.

DOI:10.1073/pnas.79.21.6424
PMID:6292900
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC347138/
Abstract

The proton markers located in the minor groove of the d(C-G-C-G-A-A-T-T-C-G-C-G) duplex and its netropsin complex have been assigned from measurements of intramolecular nuclear Overhauser effects (NOEs) between exchangeable imino protons and nonexchangeable base protons on the same and adjacent base pairs. Several points of contact between the concave face of the antibiotic and the minor groove d(A-A-T-T) tetranucleotide segment of the dodecanucleotide duplex have been established based on intermolecular NOE effects between the pyrrole ring and side-chain methylene protons of netropsin and the adenosine H-2 protons of dA X dT base pairs in the center of the duplex. These NOE measurements provide a powerful method for differentiating between minor and major groove contacts in ligand-DNA complexes in solution. A model for netropsin interaction at dA X dT sites on duplex DNA is proposed.

摘要

通过测量可交换亚氨基质子与同一碱基对及相邻碱基对中不可交换碱基质子之间的分子内核Overhauser效应（NOE），已确定了位于d（C-G-C-G-A-A-T-T-C-G-C-G）双链体及其与纺锤菌素复合物小沟中的质子标记。基于纺锤菌素的吡咯环和侧链亚甲基质子与双链体中心dA×dT碱基对的腺苷H-2质子之间的分子间NOE效应，已确定了抗生素凹面与十二核苷酸双链体小沟d（A-A-T-T）四核苷酸片段之间的几个接触点。这些NOE测量为区分溶液中配体-DNA复合物中小沟和大沟接触提供了一种有力方法。提出了双链DNA上dA×dT位点处纺锤菌素相互作用的模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c54/347138/9c053d3de02d/pnas00460-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c54/347138/38302181b3c8/pnas00460-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c54/347138/9c053d3de02d/pnas00460-0021-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c54/347138/38302181b3c8/pnas00460-0019-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5c54/347138/9c053d3de02d/pnas00460-0021-a.jpg

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本文引用的文献

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Premelting and melting transitions in the d(CGCGAATTCGCG) self-complementary duplex in solution.溶液中d(CGCGAATTCGCG)自互补双链体的预熔解和熔解转变
Biochemistry. 1982 Feb 2;21(3):428-36. doi: 10.1021/bi00532a002.
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Right-handed and left-handed DNA: studies of B- and Z-DNA by using proton nuclear Overhauser effect and P NMR.右手螺旋和左手螺旋DNA：利用质子核Overhauser效应和磷核磁共振对B型和Z型DNA的研究
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1413-7. doi: 10.1073/pnas.79.5.1413.
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Direct assignment of the dihydrouridine-helix imino proton resonances in transfer ribonucleic acid nuclear magnetic resonance spectra by means of the nuclear Overhauser effect.
Bioorg Med Chem. 2013 Oct 15;21(20):6101-14. doi: 10.1016/j.bmc.2013.04.023. Epub 2013 Apr 18.
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Polyamide-scorpion cyclam lexitropsins selectively bind AT-rich DNA independently of the nature of the coordinated metal.聚酰胺-蝎形环番利特罗品选择性地结合富含 AT 的 DNA，而与配位金属的性质无关。
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6
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