离体脊椎动物视杆光感受器中漂白适应的空间定位。
Spatial localization of bleaching adaptation in isolated vertebrate rod photoreceptors.
作者信息
Cornwall M C, Fein A, MacNichol E F
出版信息
Proc Natl Acad Sci U S A. 1983 May;80(9):2785-8. doi: 10.1073/pnas.80.9.2785.
Abstract
Bleaching of a large fraction of the rhodopsin in isolated rod outer segments results in an irreversible desensitization of the rod. This desensitization is referred to as bleaching adaptation. The logarithm of the sensitivity of the rod during bleaching adaptation has been found by a number of workers to be linearly related to the concentration of unbleached rhodopsin. We have measured the desensitization due to bleaching adaptation produced by a spatially confined stimulus and found that its effects are highly local. The space constant for the spread of desensitization was less than 4 microns. The small apparent spread of desensitization beyond the bleached regions probably can be accounted for by defocusing and light scatter. Thus, the involvement of a freely diffusible transmitter in bleaching adaptation does not appear to be required.
摘要
在分离的视杆细胞外段中，大部分视紫红质的漂白会导致视杆细胞不可逆的脱敏。这种脱敏被称为漂白适应。许多研究人员发现，在漂白适应过程中视杆细胞敏感性的对数与未漂白视紫红质的浓度呈线性关系。我们测量了由空间受限刺激产生的漂白适应所导致的脱敏，发现其效应具有高度局部性。脱敏扩散的空间常数小于4微米。脱敏在漂白区域之外的微小表观扩散可能可以通过散焦和光散射来解释。因此，似乎不需要一种可自由扩散的递质参与漂白适应。
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