离体脊椎动物视杆光感受器中漂白适应的空间定位。
Spatial localization of bleaching adaptation in isolated vertebrate rod photoreceptors.
作者信息
Cornwall M C, Fein A, MacNichol E F
出版信息
Proc Natl Acad Sci U S A. 1983 May;80(9):2785-8. doi: 10.1073/pnas.80.9.2785.
Abstract
Bleaching of a large fraction of the rhodopsin in isolated rod outer segments results in an irreversible desensitization of the rod. This desensitization is referred to as bleaching adaptation. The logarithm of the sensitivity of the rod during bleaching adaptation has been found by a number of workers to be linearly related to the concentration of unbleached rhodopsin. We have measured the desensitization due to bleaching adaptation produced by a spatially confined stimulus and found that its effects are highly local. The space constant for the spread of desensitization was less than 4 microns. The small apparent spread of desensitization beyond the bleached regions probably can be accounted for by defocusing and light scatter. Thus, the involvement of a freely diffusible transmitter in bleaching adaptation does not appear to be required.
摘要
在分离的视杆细胞外段中，大部分视紫红质的漂白会导致视杆细胞不可逆的脱敏。这种脱敏被称为漂白适应。许多研究人员发现，在漂白适应过程中视杆细胞敏感性的对数与未漂白视紫红质的浓度呈线性关系。我们测量了由空间受限刺激产生的漂白适应所导致的脱敏，发现其效应具有高度局部性。脱敏扩散的空间常数小于4微米。脱敏在漂白区域之外的微小表观扩散可能可以通过散焦和光散射来解释。因此，似乎不需要一种可自由扩散的递质参与漂白适应。
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本文引用的文献
1
NEURAL AND PHOTOCHEMICAL MECHANISMS OF VISUAL ADAPTATION IN THE RAT.大鼠视觉适应的神经和光化学机制
J Gen Physiol. 1963 Jul;46(6):1287-301. doi: 10.1085/jgp.46.6.1287.
2
Rhodopsin measurement and dark-adaptation in a subject deficient in cone vision.在一名缺乏视锥细胞视觉的受试者中进行视紫红质测量和暗适应。
J Physiol. 1961 Apr;156(1):193-205. doi: 10.1113/jphysiol.1961.sp006668.
3
Chemistry of visual adaptation in the rat.大鼠视觉适应的化学过程
Nature. 1960 Oct 8;188:114-8. doi: 10.1038/188114a0.
4
Local effects of bleaching in retinal rods of the toad.蟾蜍视网膜视杆细胞中漂白的局部效应。
J Physiol. 1982 Jul;328:49-71. doi: 10.1113/jphysiol.1982.sp014252.
5
Longitudinal spread of adaptation in the rods of the frog's retina.青蛙视网膜视杆细胞中适应性的纵向传播。
J Physiol. 1981 Jan;310:501-28. doi: 10.1113/jphysiol.1981.sp013564.
6
Spatial spread of activation and background desensitization in toad rod outer segments.蟾蜍视杆细胞外段激活的空间扩散及背景脱敏
J Physiol. 1981;319:463-96. doi: 10.1113/jphysiol.1981.sp013921.
7
Light and dark adaptation in the isolated rat retina.离体大鼠视网膜的明适应和暗适应
Nature. 1967 Jul 8;215(5097):134-8. doi: 10.1038/215134a0.
8
Properties of "neural" adaptation in components of the frog electroretinogram.青蛙视网膜电图各成分中“神经”适应的特性
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Vision Res. 1972 Dec;12(12):1937-46. doi: 10.1016/0042-6989(72)90050-8.
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