Simmet T, Jaffe B M
Prostaglandins. 1983 Jan;25(1):47-54. doi: 10.1016/0090-6980(83)90134-x.
Prostaglandin D2 was found to be a potent inhibitor of B-16 melanoma cell replication in vitro. The inhibition was dose-dependent between 3x10(-9)M and 3x10(-6)M (IC50 approximately 0.3 microM after 6 days). On a molar basis, PGD2 was a better inhibitor than PGA2 or 16, 16-dimethyl-PGE2-methyl ester (di-M-PGE2) and in higher concentrations (10(-6)-10(-7)M), comparable to retinoic acid. In higher concentrations, PGD2 inhibited DNA, RNA and protein synthesis. The B-16 melanoma cell line which we used synthesized arachidonic acid metabolites which comigrated with PGA2, PGD2, PGE2, and PGF2 alpha on a thin layer chromatography system.
前列腺素D2被发现是体外B - 16黑色素瘤细胞复制的有效抑制剂。在3×10⁻⁹M至3×10⁻⁶M之间，抑制作用呈剂量依赖性（6天后IC50约为0.3微摩尔）。按摩尔计算，PGD2比PGA2或16,16 - 二甲基 - PGE2甲酯（二 - M - PGE2）是更好的抑制剂，在较高浓度（10⁻⁶ - 10⁻⁷M）下，与视黄酸相当。在较高浓度下，PGD2抑制DNA、RNA和蛋白质合成。我们使用的B - 16黑色素瘤细胞系在薄层色谱系统上合成了与PGA2、PGD2、PGE2和PGF2α共迁移的花生四烯酸代谢物。
