Yoshida T, Ohki S, Kanazawa M, Mizunuma H, Kikuchi Y, Satoh H, Andoh Y, Tsuchiya A, Abe R
Second Department of Surgery, Fukushima Medical College, Japan.
Surg Today. 1998;28(7):740-5. doi: 10.1007/BF02484622.
The inhibitory action of prostaglandin D2 (PGD2) and its effect on the cell cycle were examined in cell lines SW480 and LS174T of human colon cancer. The growth of the cell lines were assessed 24 h and 48 h after the addition of 1.0 microgram/ml and 10.0 micrograms/ml PGD2. The growth of SW480 cells was inhibited 48 h, but not 24 h, after the addition of 1.0 microgram/ml, and 24 h and 48 h after the addition of 10.0 micrograms/ml, while that of LS174T was inhibited by both doses after 24 h and 48 h. S-Phase DNA synthesis in the SW480 cells was significantly blocked 24 h after the addition of 10.0 micrograms/ml PGD2. The cell cycle of LS174T cells was arrested at the G0 + G1 phase 24 h after the addition of 1.0 microgram/ml and 10.0 micrograms/ml PGD2. The correlation between hepatic metastasis and PGD2 concentration in human cancer tissue was examined. The mean value of PGD2 concentrations in the primary cancer tissue was significantly lower in the hepatic metastasis group than that in the group without hepatic metastasis. These findings suggest that measuring the PGD2 in cancer tissue may be useful for detecting and predicting the hepatic metastasis from human colorectal cancer.
在人结肠癌的SW480和LS174T细胞系中检测了前列腺素D2（PGD2）的抑制作用及其对细胞周期的影响。在添加1.0微克/毫升和10.0微克/毫升PGD2后24小时和48小时评估细胞系的生长情况。添加1.0微克/毫升PGD2后48小时，SW480细胞的生长受到抑制，但24小时时未受抑制；添加10.0微克/毫升PGD2后24小时和48小时，其生长均受到抑制，而LS174T细胞在添加这两种剂量的PGD2后24小时和48小时生长均受到抑制。添加10.0微克/毫升PGD2后24小时，SW480细胞中的S期DNA合成被显著阻断。添加1.0微克/毫升和10.0微克/毫升PGD2后24小时，LS174T细胞的细胞周期停滞在G0 + G1期。检测了人癌组织中肝转移与PGD2浓度之间的相关性。肝转移组原发癌组织中PGD2浓度的平均值显著低于无肝转移组。这些发现表明，检测癌组织中的PGD2可能有助于检测和预测人结直肠癌的肝转移。
