Weinberg J B, Haney A F
J Natl Cancer Inst. 1983 Jun;70(6):1005-10.
Human mononuclear phagocytes (monocytes and macrophages) act as effectors in the destruction of tumor cells. Peritoneal macrophages from normal or infertile women killed a variety of tumor cells in vitro more efficiently than did blood monocytes from the same subjects. Lysis depended on the effector-to-target cell ratio and was neither reproduced by supernatants from nor lysates of the mononuclear phagocytes. Normal fibroblasts were not lysed. Lipopolysaccharide (10(1)-10(4) ng/ml) did not alter the monocyte- or macrophage-mediated tumor cell killing. The monocytes and macrophages had equivalent basal and phorbol 12,13-myristate acetate-stimulated H2O2 and O-2 production, and the reactive oxygen species scavengers or quenchers catalase, superoxide dismutase, mannitol, and L-histidine did not diminish the killing. These observations suggest that the spontaneous tumor cell killing by human mononuclear phagocytes was not mediated by reactive oxygen species.
人类单核吞噬细胞（单核细胞和巨噬细胞）在肿瘤细胞的破坏过程中发挥效应作用。来自正常或不孕女性的腹腔巨噬细胞在体外杀死多种肿瘤细胞的效率高于同一受试者的血液单核细胞。细胞溶解取决于效应细胞与靶细胞的比例，单核吞噬细胞的上清液或裂解物均不能重现这种溶解现象。正常成纤维细胞不会被溶解。脂多糖（10¹ - 10⁴ ng/ml）不会改变单核细胞或巨噬细胞介导的肿瘤细胞杀伤作用。单核细胞和巨噬细胞在基础状态以及佛波酯12,13 - 肉豆蔻酸乙酸酯刺激下产生过氧化氢和超氧阴离子的能力相当，活性氧清除剂或淬灭剂过氧化氢酶、超氧化物歧化酶、甘露醇和L - 组氨酸不会减弱杀伤作用。这些观察结果表明，人类单核吞噬细胞对肿瘤细胞的自发杀伤作用不是由活性氧介导的。
