Lacaze N, Gombaud-Saintonge G, Lanotte M
Leuk Res. 1983;7(2):145-54. doi: 10.1016/0145-2126(83)90005-x.
Conditions for in vitro long-term maintenance and proliferation of the Brown Norway (BN) rat myelocytic leukemia cell (BNML) are described. During a primary culture of leukemic rat marrow, a few leukemic cells proliferated and were initially dependent on an adherent cell population but later acquired the capability of independent growth. A wild BN leukemic stem cell line has been maintained in vitro for several months, without noticeable phenotypic alterations. The doubling time of the cultured cells was 40 h. The cells were promyelocytes. The cytochemical markers of the original BN leukemia cells were preserved. The cultured cell line transferred leukemia exclusively to BN rats. Wistar and BDIX rats were resistant. The virulence of cultured leukemic cell was measured by shortened survival times after transplantation in animals of a fixed number of leukemic cells. The role of bone marrow microenvironment in the initiation of long-term growth is discussed.
描述了体外长期维持和增殖棕色挪威（BN）大鼠骨髓细胞白血病细胞（BNML）的条件。在白血病大鼠骨髓的原代培养过程中，少数白血病细胞增殖，最初依赖贴壁细胞群体，但后来获得了独立生长的能力。一种野生型BN白血病干细胞系已在体外维持了数月，没有明显的表型改变。培养细胞的倍增时间为40小时。这些细胞是早幼粒细胞。原始BN白血病细胞的细胞化学标志物得以保留。培养的细胞系仅将白血病转移至BN大鼠。Wistar和BDIX大鼠具有抗性。通过将固定数量的白血病细胞移植到动物体内后缩短的存活时间来测量培养的白血病细胞的毒力。讨论了骨髓微环境在长期生长起始中的作用。
