Lanotte M, Lacaze N, Gombaud-Saintonge G
Leuk Res. 1984;8(1):71-80. doi: 10.1016/0145-2126(84)90033-x.
We report a reproducible in vitro clonogenic assay for the transplantable BN rat promyelocytic leukemia (BNML). Colony growth required a feeder activity elaborated by normal rat marrow cells. This stimulating activity is ascribed to the stromal elements. The in vitro maintained BNML cell line IPC-81 [Lacaze et al., Leukemia Research 7, 145 (1983)] also exhibited stimulating activities at high cell concentrations, confirming the autocrine capacities previously described. The nature of the stimulating activity is unknown but it is probably not of CSF type, and is not transferred to culture supernatants. This in vitro clonal assay permits the quantification of the clonogenic cells present in leukemic marrow during the early stage of the disease, when BNML cells are not yet distinguishable morphologically. Leukemic Cell Forming Unit (L-CFU) response was linear; 5-10(3) clones can be scored reproducibly. The plating efficiency obtained with cultured IPC-81 cells was high (60-90%), whereas marrow transplanted leukemia cells had reduced clonogenic capacities. These results are discussed.
我们报道了一种可重复的体外克隆形成试验，用于可移植的BN大鼠早幼粒细胞白血病（BNML）。集落生长需要正常大鼠骨髓细胞产生的饲养层活性。这种刺激活性归因于基质成分。体外培养的BNML细胞系IPC-81 [Lacaze等人，《白血病研究》7，145（1983）] 在高细胞浓度下也表现出刺激活性，证实了先前描述的自分泌能力。刺激活性的性质尚不清楚，但可能不是CSF类型，也不会转移到培养上清液中。这种体外克隆试验能够在疾病早期对白血病骨髓中存在的克隆形成细胞进行定量，此时BNML细胞在形态上还无法区分。白血病细胞形成单位（L-CFU）反应呈线性；可重复计数5 - 10(3)个克隆。培养的IPC-81细胞的接种效率很高（60 - 90%），而骨髓移植的白血病细胞的克隆形成能力则降低。对这些结果进行了讨论。
