Coulombel L, Kalousek D K, Eaves C J, Gupta C M, Eaves A C
N Engl J Med. 1983 Jun 23;308(25):1493-8. doi: 10.1056/NEJM198306233082502.
We found that when marrow cells from four patients with newly diagnosed Philadelphia chromosome-positive chronic myelogenous leukemia were maintained in culture for two to four weeks, a previously undetectable population of chromosomally normal hematopoietic cells (including erythroid, granulopoietic, and pluripotent progenitors) became readily demonstrable in three cases. Time-course studies showed that in such cultures the dominant Philadelphia chromosome-positive population rapidly disappeared, in contrast to coexisting chromosomally normal progenitors, which remained detectable for periods of two to three months. Long-term marrow cultures thus offer a new approach to the assessment of a suppressed but functionally intact population of chromosomally normal hematopoietic stem cells in patients with chronic myelogenous leukemia.
我们发现，将4例新诊断的费城染色体阳性慢性粒细胞白血病患者的骨髓细胞在培养中维持两到四周后，在3例患者中，先前无法检测到的染色体正常的造血细胞群体（包括红系、粒系和多能祖细胞）变得易于检测到。时间进程研究表明，在这样的培养物中，占主导的费城染色体阳性群体迅速消失，与之形成对比的是，共存的染色体正常祖细胞在两到三个月的时间里仍可检测到。因此，长期骨髓培养为评估慢性粒细胞白血病患者中受抑制但功能完整的染色体正常造血干细胞群体提供了一种新方法。
