Charnay P, Maniatis T
Science. 1983 Jun 17;220(4603):1281-3. doi: 10.1126/science.6574602.
The effect of hemin on the rate of synthesis and the level of globin messenger RNA's in the human erythroid cell line K562 was examined by means of cloned hybridization probes specific for each of the human embryonic, fetal, and adult globin genes. Hemin increases both the rate of transcription and the level of accumulation of zeta-, epsilon-, gamma-, and alpha-globin messenger RNA's by a factor of 3 to 5. Thus, hemin induction of globin gene expression in K562 cells is at the level of transcription.
通过针对人类胚胎、胎儿和成人珠蛋白基因的克隆杂交探针，研究了氯化血红素对人红系细胞系K562中珠蛋白信使RNA合成速率和水平的影响。氯化血红素使ζ、ε、γ和α珠蛋白信使RNA的转录速率和积累水平均提高了3至5倍。因此，氯化血红素对K562细胞中珠蛋白基因表达的诱导作用发生在转录水平。
