在主要产生血红蛋白戈尔1的人白血病K562细胞中，高效的细胞增殖和ε-珠蛋白mRNA的主要积累。
Efficient cell proliferation and predominant accumulation of epsilon-globin mRNA in human leukemic K562 cells which produce mostly Hb Gower 1.
作者信息
Gambari R, Amelotti F, Piva R
出版信息
Experientia. 1985 May 15;41(5):673-5. doi: 10.1007/BF02007714.
Long-term cultures of K562(S) cells in 50-75 microM hemin allow the selection of 'hemin-resistant' K562 cells together with cells which proliferate efficiently while fully induced to express the human embryonic globin genes, as the hemoglobin Gower 1 (zeta 2 epsilon 2) is the predominant hemoglobin produced. Our experiments demonstrate that these K562 cells accumulate mostly epsilon-globin mRNA (epsilon-globin mRNA/gamma-globin mRNA = 2.9) suggesting that the control of hemoglobin expression is at a pretranslational level.
在含有50-75微摩尔血红素的条件下对K562(S)细胞进行长期培养，能够筛选出“抗血红素”的K562细胞，同时还能筛选出在被充分诱导表达人类胚胎珠蛋白基因时高效增殖的细胞，因为血红蛋白戈尔1（ζ2ε2）是产生的主要血红蛋白。我们的实验表明，这些K562细胞主要积累ε-珠蛋白mRNA（ε-珠蛋白mRNA/γ-珠蛋白mRNA = 2.9），这表明血红蛋白表达的控制处于翻译前水平。
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