Grossman E S, Austin J C
Arch Oral Biol. 1983;28(3):279-81. doi: 10.1016/0003-9969(83)90159-0.
Pulp tissue in the incisors was fixed in situ by perfusion. Cervical thirds of the tooth crowns were excised by sectioning the teeth transversely and cryofractured through the long axis of the tooth in a bucco-lingual mid-axial plane to expose the dentine from the pulp to the enamel-dentine junction. Specimens were freeze-dried and the fracture surfaces examined by scanning electron microscopy. Flattened tubular processes were noted protruding from the open ends of transversely fractured dentinal tubules in the outer third of the dentine. These structures were present in fractured tubules to within 20 microns of the enamel-dentine junction. These observations suggest that odontoblast processes traverse the full length of dentinal tubules.
切牙的牙髓组织通过灌注进行原位固定。将牙冠的颈部三分之一横向切片切除,然后沿牙齿长轴在颊舌中轴平面进行冷冻断裂,以暴露从牙髓到釉质-牙本质界的牙本质。标本经冷冻干燥后,通过扫描电子显微镜检查断裂面。注意到在牙本质外层三分之一处,横向断裂的牙本质小管开口端有扁平的管状突起。这些结构存在于距釉质-牙本质界20微米范围内的断裂小管中。这些观察结果表明,成牙本质细胞突起贯穿牙本质小管的全长。