Sasaki Y, Sakihama T, Kamikubo T, Shinozaki K
Eur J Biochem. 1983 Jul 1;133(3):617-20. doi: 10.1111/j.1432-1033.1983.tb07507.x.
Etiolated pea seedlings (5 days old) were treated with red light for 5 min and grown for 2 days more in darkness. Effects of the red light on the mRNA levels of two subunits of ribulose-1,5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39) (ribulose-P2 carboxylase), rate of enzyme synthesis and accumulation of the enzyme were examined for the plants. Increases of the two mRNA levels, encoded in nuclei and chloroplasts were observed. Red light followed by far-red light treatment for 5 min inhibited the increases. An increase parallel with the mRNA levels was found for the rate of the enzyme synthesis and accumulation of the enzyme. These results indicate that the induction of two mRNA levels is mediated by phytochrome and causes the red-light-inducible, far-red-light-reversible increase of ribulose-P2 carboxylase. When white light was irradiated to induce the enzyme, the effects of red light given in darkness were reinforced. These results indicate that light-induction of ribulose-P2 carboxylase is mediated by phytochrome and controlled by the two mRNA levels.
将黄化豌豆幼苗（5日龄）用红光处理5分钟，然后在黑暗中再培养2天。检测了红光对植物中核酮糖-1,5-二磷酸羧化酶/加氧酶（EC 4.1.1.39）（核酮糖-P2羧化酶）两个亚基的mRNA水平、酶合成速率和酶积累的影响。观察到细胞核和叶绿体中编码的两种mRNA水平均增加。红光后接着用远红光处理5分钟抑制了这种增加。发现酶合成速率和酶积累与mRNA水平平行增加。这些结果表明，两种mRNA水平的诱导是由光敏色素介导的，并导致核酮糖-P2羧化酶的红光诱导、远红光可逆增加。当照射白光诱导该酶时，黑暗中给予的红光的作用得到增强。这些结果表明，核酮糖-P2羧化酶的光诱导是由光敏色素介导的，并受两种mRNA水平的控制。
