Grotjan H E, Steinberger E
Clin Chem. 1978 Jul;24(7):1193-5.
A major expense of radioimmunoassay involving tritiated tracers is that of liquid scintillation counting. We present a micro-scale method that markedly reduces the cost of liquid scintillation counting. The radioimmunoassay is done in 10 X 50 mm tubes. The antibody-bound tracer is precipitated with a second antibody, the precipitate is resuspended in 0.1 ml of water, and 0.3 ml of dioxane is added. One milliliter of a toluene-based scintillation cocktail is added, and the tube is capped and placed in an adapter for liquid scintilation counting. When this method was applied to a double-antibody testosterone radioimmunoassay, it performed comparably to assays counted by the usual method.
涉及氚化示踪剂的放射免疫分析的一项主要费用是液体闪烁计数的费用。我们提出了一种微尺度方法，可显著降低液体闪烁计数的成本。放射免疫分析在10×50毫米的试管中进行。与抗体结合的示踪剂用第二抗体沉淀，沉淀物重悬于0.1毫升水中，然后加入0.3毫升二氧六环。加入1毫升甲苯基闪烁鸡尾酒，盖上试管盖，放入用于液体闪烁计数的适配器中。当将此方法应用于双抗体睾酮放射免疫分析时，其性能与用常规方法计数的分析相当。
