用于乙型肝炎相关抗原（HBsAg）放射免疫测定的液体闪烁计数溶液的评估。

Evaluation of liquid scintillation counting solutions for radioimmunoassay of hepatitis-associated antigen (HBsAg).

作者信息

Hicks E J, Oei T O, Nordschow C D, Hughes B J

出版信息

Transfusion. 1977 Sep-Oct;17(5):503-8. doi: 10.1046/j.1537-2995.1977.17578014592.x.

DOI:10.1046/j.1537-2995.1977.17578014592.x

Abstract

Liquid scintillation counting solutions for radioimmunoassay of hepatitis associated antigen (HBsAg) are evaluated and discussed. Data is also presented which indicates that liquid scintillation counting could be carried out by placing cut-off Ausria-125 test tubes in counting vials containing 10 ml of either Brays, Unogel, or Instagel solutions. The data suggest that Brays was the least effective and Unogel and Instagel were the most effective liquid scintillation counting solutions. This is a fundamental consideration if a laboratory is contemplating an initial investiment in counting equipment. The acquisition of a liquid scintillation counter provides additional capabilities for the clinical laboratory interested in radioisotope measurements. In addition, if a laboratory already has a liquid scintillation counter (Beta counter), the director of the clinical laboratory may elect not to purchase additional counting equipment (gamma counter) since he already has the added advantage of measuring iodine-125 with the Beta counter. The authors propose liquid scintillation (RIA) as an alternative counting system for HBsAg detection in hospital patients and donors.

摘要

对用于肝炎相关抗原（HBsAg）放射免疫分析的液体闪烁计数溶液进行了评估和讨论。还给出了数据，这些数据表明，通过将截断的Ausria - 125试管放入装有10毫升Brays、Unogel或Instagel溶液的计数瓶中，可以进行液体闪烁计数。数据表明，Brays是效果最差的液体闪烁计数溶液，而Unogel和Instagel是最有效的。如果实验室正在考虑对计数设备进行初始投资，这是一个基本的考虑因素。购置液体闪烁计数器为对放射性同位素测量感兴趣的临床实验室提供了额外的能力。此外，如果实验室已经有一台液体闪烁计数器（β计数器），临床实验室主任可能会选择不购买额外的计数设备（γ计数器），因为他已经具有用β计数器测量碘 - 125的额外优势。作者提议将液体闪烁（放射免疫分析）作为医院患者和献血者中HBsAg检测的替代计数系统。