Thébault A, Lefèvre B, Testart J
J Reprod Fertil. 1983 Jul;68(2):419-24. doi: 10.1530/jrf.0.0680419.
Ovulation was never observed, even in the presence of gonadotrophins, when preovulatory follicles isolated from oestrous does were cultured in vitro (n = 112). If the follicles were co-cultured with pieces of ovarian tissue and gonadotrophins, then 50% ovulated (P less than 0.01). Attempts to replace the ovarian tissue by various steroid hormones or prostaglandins did not result in follicular rupture except when PGE-2 was used (23.8%, P less than 0.05). We suggest that gonadotrophins have an immediate (less than 1 h) action upon the follicle and ovarian interstitial tissue simultaneously. The response of the ovarian tissue to gonadotrophins is essential for eventual follicular rupture and involves PGE-2.
即使存在促性腺激素，当从发情期母羊分离出的排卵前卵泡在体外培养时（n = 112），从未观察到排卵现象。如果卵泡与卵巢组织块和促性腺激素共同培养，则50%会排卵（P < 0.01）。尝试用各种甾体激素或前列腺素替代卵巢组织均未导致卵泡破裂，除非使用PGE - 2（23.8%，P < 0.05）。我们认为促性腺激素对卵泡和卵巢间质组织同时具有即时（< 1小时）作用。卵巢组织对促性腺激素的反应对于最终的卵泡破裂至关重要，且涉及PGE - 2。
