Testart J, Thébault A, Lefèvre B
J Reprod Fertil. 1983 Jul;68(2):413-8. doi: 10.1530/jrf.0.0680413.
Follicular rupture was never observed when preovulatory rabbit follicles were isolated from the ovary before the ovulatory gonadotrophin discharge and placed in hormone-free culture for 10-14 h (n = 48). However, if the follicles were taken 1 h post coitum and cultured under the same conditions, 11/24 (45.8%) ruptured spontaneously. Follicular rupture under these conditions appeared to be chronologically and histologically comparable to that of ovulation in vivo. Culture in the presence of progesterone increased the proportion of follicles ovulating in vitro (17/23 = 73.9%; P less than 0.05). Inhibition in vitro of the synthesis of steroids or prostaglandins suppressed ovulation, although meiotic maturation of the oocyte did take place. These findings indicate that the follicle constitutes an independent entity within the ovary from 1 h post coitum.
在排卵前促性腺激素释放之前从卵巢中分离出排卵前的兔卵泡，并在无激素培养条件下培养10 - 14小时，从未观察到卵泡破裂（n = 48）。然而，如果在交配后1小时取出卵泡并在相同条件下培养，24个中有11个（45.8%）自发破裂。在这些条件下的卵泡破裂在时间和组织学上似乎与体内排卵相当。在孕酮存在下培养增加了体外排卵的卵泡比例（17/23 = 73.9%；P小于0.05）。体外抑制类固醇或前列腺素的合成可抑制排卵，尽管卵母细胞的减数分裂成熟确实发生了。这些发现表明，从交配后1小时起，卵泡在卵巢内构成一个独立的实体。
