van Lente F, Galen R S
Clin Chim Acta. 1978 Jul 15;87(2):211-7. doi: 10.1016/0009-8981(78)90340-6.
A procedure is described for accurately determining the presence of true creatine kinase isoenzyme BB by fluorescent staining following electrophoresis on either cellulose acetate or agarose. Treatment of sera with anti-BB inhibiting antibody prior to electrophoresis and subsequent enzymatic staining allows clear identification of creatine kinase BB in presence of interfering artifacts. The utilization of an immunological means of identifying BB following electrophoresis avoids the pitfall of associating an artifactual BB isoenzyme with a disease state. Using this technique, presence of creatine kinase BB in patients with carcinoma, cardiac disease and renal failure has been verified.
本文描述了一种通过在醋酸纤维素或琼脂糖上进行电泳后进行荧光染色来准确测定真肌酸激酶同工酶BB存在的方法。在电泳前用抗BB抑制抗体处理血清并随后进行酶染色，可在存在干扰假象的情况下清晰鉴定肌酸激酶BB。电泳后利用免疫学方法鉴定BB可避免将假象性BB同工酶与疾病状态相关联的陷阱。使用该技术，已证实癌症、心脏病和肾衰竭患者中存在肌酸激酶BB。
