Stephen J, Ambrose E J
Acta Cytol. 1983 May-Jun;27(3):362-4.
Primary cultures of human breast carcinoma cells were treated for 24 hours with different concentrations of anticancer drugs followed by labelling with 3H-thymidine and 3H-uridine and subsequent autoradiographic processing to study the effect of drugs on nucleic acid synthesis in these cells. 3H-uridine autoradiography was more sensitive than 3H-thymidine autoradiography in the assessment of lethal effects. The results showed that individual neoplasms varied in drug response. This method may be useful for predicting the effectiveness of drugs for treatment of individual cases and also for screening new drugs for anticancer activity.
人乳腺癌细胞原代培养物用不同浓度的抗癌药物处理24小时，然后用³H-胸腺嘧啶核苷和³H-尿苷进行标记，随后进行放射自显影处理，以研究药物对这些细胞核酸合成的影响。在评估致死效应方面，³H-尿苷放射自显影比³H-胸腺嘧啶核苷放射自显影更敏感。结果表明，不同肿瘤对药物的反应各异。该方法可能有助于预测药物对个别病例治疗的有效性，也可用于筛选具有抗癌活性的新药。
