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[吞噬作用期间细菌核酸生物合成的电子放射自显影研究方法]

[Method of electron-autoradiographic study of nucleic acid biosynthesis in bacteria during phagocytosis].

作者信息

Pal'tsyn A A, Pobedina V G, Chervonskaia N V, Badikova A K, Sanovich E Ia

出版信息

Biull Eksp Biol Med. 1981 Jun;91(6):763-5.

PMID:6168317
Abstract

Leukocytes from healthy men exposed to Staphylococcus epidermis were incubated with 3H-uridine and 3H-thymidine. The level of nucleic acid synthesis by phagocyted bacteria was examined by electron microscopic autoradiography. The method makes it possible to correlate bacterial and phagocyte functions with their ultrastructures.

摘要

将来自接触表皮葡萄球菌的健康男性的白细胞与3H-尿苷和3H-胸苷一起培养。通过电子显微镜放射自显影术检测吞噬细菌的核酸合成水平。该方法能够将细菌和吞噬细胞的功能与其超微结构联系起来。

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