在无血清且添加激素的培养基中培养人骨肉瘤细胞系。

Cultivation of human osteosarcoma cell lines in serum-free hormone supplemented medium.

作者信息

Tsang K Y, Fudenberg H H, Sun D C, Pai G S, Bishop L R, Sager S

出版信息

In Vitro. 1983 Jul;19(7):515-21. doi: 10.1007/BF02619599.

DOI:10.1007/BF02619599

Abstract

Two human osteosarcoma cell lines (TE-85, LM-1) were adapted to grow in serum-free hydrocortisone supplemented medium. Human osteosarcoma associated antigens were detected both on the cell membrane as well as in the concentrated culture medium. TE-85 cells produced bone specific alkaline phosphatase and LM-1 cells produced both bone specific and placental-like alkaline phosphatases when cultured in this medium. Successful proliferation of human osteosarcoma cell lines in serum-free medium is valuable in human osteosarcoma research.

摘要

两种人骨肉瘤细胞系（TE - 85、LM - 1）被培养适应于在添加了氢化可的松的无血清培养基中生长。在细胞膜以及浓缩的培养基中均检测到了人骨肉瘤相关抗原。当在这种培养基中培养时，TE - 85细胞产生骨特异性碱性磷酸酶，而LM - 1细胞则产生骨特异性和胎盘样碱性磷酸酶。人骨肉瘤细胞系在无血清培养基中的成功增殖在人骨肉瘤研究中具有重要价值。