Marceau N, Noël M, Deschênes J
In Vitro. 1982 Jan;18(1):1-11. doi: 10.1007/BF02796379.
Hepatocytes isolated from neonatal (NN) and adult (AD) rats were seeded on fibronectin coated substratum and cultured in arginine-free medium supplemented with various combinations of insulin, dexamethasone, triiodothyronine (T3), albumin, and transferrin, in presence or absence of fibronectin depleted serum (FDS). The main finding is that in response to certain hormone mixtures, both NN and AD hepatocytes can be stimulated to proliferate, as revealed by an increase in cell number, a [3H]thymidine incorporation into nuclei, and extractable DNA as well as the appearance of mitotic figures. Moreover, this proliferative activity is associated with changes in hepatocyte ploidy. However, the proliferative response of NN hepatocytes to hormone action is much different from that of AD hepatocytes, and the addition of FDS amplifies this activity in NN but inhibits it in AD hepatocyte cultures. Measurements of tyrosine aminotransferase and lactate dehydrogenase activities indicate a good preservation of NN and AD hepatocyte functional integrity under certain culture conditions. A good maintenance of albumin production in NN and AD hepatocyte cultures requires the presence of dexamethasone, whereas the alpha-fetoprotein production in NN hepatocyte cultures is reduced quite rapidly under most conditions. No alpha-fetoprotein is detectable in AD hepatocyte cultures.
从新生（NN）和成年（AD）大鼠分离的肝细胞接种于纤连蛋白包被的基质上，并在无精氨酸培养基中培养，该培养基添加了胰岛素、地塞米松、三碘甲状腺原氨酸（T3）、白蛋白和转铁蛋白的各种组合，同时存在或不存在纤连蛋白缺失血清（FDS）。主要发现是，在某些激素混合物的作用下，NN和AD肝细胞均可被刺激增殖，这可通过细胞数量增加、[3H]胸腺嘧啶核苷掺入细胞核、可提取DNA以及有丝分裂相的出现来证明。此外，这种增殖活性与肝细胞倍性的变化有关。然而，NN肝细胞对激素作用的增殖反应与AD肝细胞有很大不同，添加FDS可增强NN肝细胞的这种活性，但在AD肝细胞培养中则抑制其活性。酪氨酸转氨酶和乳酸脱氢酶活性的测定表明，在某些培养条件下，NN和AD肝细胞的功能完整性得到了良好的保存。NN和AD肝细胞培养中白蛋白的良好产生需要地塞米松的存在，而在大多数情况下，NN肝细胞培养中甲胎蛋白的产生会迅速减少。在AD肝细胞培养中未检测到甲胎蛋白。
