Elattar T M, Lin H S, Tira D E
J Dent Res. 1983 Sep;62(9):975-9. doi: 10.1177/00220345830620091201.
We investigated the effect of non-steroidal anti-inflammatory drugs on prostaglandins (PGs) and 12-hydroxyeicosatetraenoic acid (12-HETE) formation by inflamed human gingival tissues. Gingival tissue homogenates were incubated with 14C-arachidonic acid in the presence of indomethacin, piroxicam, or ibuprofen, and the organic solvent extracts were chromatographed on silica gel plates with standards for radiometric assay. There was a significant negative trend between the doses (10(-7)-10(-3) M) of each of indomethacin, piroxicam, and ibuprofen, and the amounts of PGF2 alpha, PGE2, PGD2, and 15-keto-PGE2 produced. All three drugs have a significant inhibitory effect on PGs and 12-HETE production at 10(-3) M when compared with the control. The rank order effectiveness of the drugs, at 10(-3) M, on PG inhibition was indomethacin greater than piroxicam greater than ibuprofen, and on 12-HETE inhibition was indomethacin greater than ibuprofen greater than piroxicam.
我们研究了非甾体抗炎药对人炎症牙龈组织中前列腺素（PGs）和12-羟基二十碳四烯酸（12-HETE）生成的影响。将牙龈组织匀浆与14C-花生四烯酸在吲哚美辛、吡罗昔康或布洛芬存在的情况下孵育，然后将有机溶剂提取物在硅胶板上进行色谱分析，并与标准品一起进行放射性测定。吲哚美辛、吡罗昔康和布洛芬各自的剂量（10^(-7)-10^(-3) M）与所产生的前列腺素F2α（PGF2α）、前列腺素E2（PGE2）、前列腺素D2（PGD2）和15-酮基前列腺素E2（15-keto-PGE2）的量之间存在显著的负相关趋势。与对照组相比，所有三种药物在10^(-3) M时对PGs和12-HETE的产生均有显著抑制作用。在10^(-3) M时，这些药物对PG抑制的有效性排序为：吲哚美辛＞吡罗昔康＞布洛芬；对12-HETE抑制的有效性排序为：吲哚美辛＞布洛芬＞吡罗昔康。
