Rosenblum B B, Neel J V, Hanash S M
Proc Natl Acad Sci U S A. 1983 Aug;80(16):5002-6. doi: 10.1073/pnas.80.16.5002.
A series of 62 plasma samples have been examined for genetic variation by the technique of two-dimensional polyacrylamide gel electrophoresis followed by silver-staining of the gels. Twenty polypeptides chosen without respect to variability were considered suitable for scoring. Of the total of 1,240 polypeptides, 29 could not be scored unambiguously. Seventy-five of the remaining 1,211 exhibited the combination of a normal and a variant polypeptide. All variants were present in either the father or the mother of the subject. This index of heterozygosity (6.2 +/- 0.7%) is substantially higher than those reported by others in similar studies of human fibroblasts, lymphocytes, kidney, or brain cells.
通过二维聚丙烯酰胺凝胶电泳技术，随后对凝胶进行银染，对62份血浆样本进行了遗传变异检测。选择了20种不考虑变异性的多肽用于评分。在总共1240种多肽中，有29种无法明确评分。其余1211种多肽中有75种表现出正常多肽和变异多肽的组合。所有变异都存在于受试者的父亲或母亲中。这种杂合度指数（6.2 +/- 0.7%）显著高于其他在人类成纤维细胞、淋巴细胞、肾脏或脑细胞的类似研究中报告的指数。
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