Kedar I, Jacob E T, Bar-Natan N, Ravid M
Ann N Y Acad Sci. 1983;411:131-4. doi: 10.1111/j.1749-6632.1983.tb47294.x.
Renal ischemia was produced in rats by clamping of the renal artery for 1 h. Upon termination of the ischemic period a 20% solution of DMSO (5 g kg-1 b.w.) was given intravenously to 33 rats. Eighteen control animals received normal saline. All DMSO-treated animals survived while all control animals died within the subsequent seven days. At 24 h following the experiment, the mean blood urea of the control rats was 254 mg/100 ml and the mean plasma creatinine 7.2 mg/100 ml. By contrast, the DMSO-treated rats had a mean blood urea of 69 mg/100 ml and plasma creatinine of 1.6 mg/100 ml. In 17 animals the kidney was perfused with DMSO prior to the closure of the renal artery. All these rats survived the procedure and showed near normal kidney function at 24 h. The renal artery was clamped for 60 min in ten dogs. Five dogs received DMSO (3 g kg-1 b.w.) and the other five received an equivalent dose of normal saline. Three weeks later a contralateral nephrectomy was performed. Renal function was normal in the DMSO-treated dogs. One control dog died of uremia, in the remaining four a transient renal failure was observed. These experiments in two different animals highlight the protective effect of DMSO on the ischemic kidney when the drug is administered after the ischemic period.
通过夹闭大鼠肾动脉1小时来制造肾缺血。在缺血期结束时，给33只大鼠静脉注射20%的二甲基亚砜溶液（5克/千克体重）。18只对照动物接受生理盐水。所有经二甲基亚砜处理的动物均存活，而所有对照动物在随后的七天内死亡。实验后24小时，对照大鼠的平均血尿素为254毫克/100毫升，平均血浆肌酐为7.2毫克/100毫升。相比之下，经二甲基亚砜处理的大鼠平均血尿素为69毫克/100毫升，血浆肌酐为1.6毫克/100毫升。在17只动物中，在关闭肾动脉之前用二甲基亚砜灌注肾脏。所有这些大鼠在该手术后存活，并且在24小时时显示出接近正常的肾功能。在10只狗中夹闭肾动脉60分钟。5只狗接受二甲基亚砜（3克/千克体重），另外5只接受等量的生理盐水。三周后进行对侧肾切除术。经二甲基亚砜处理的狗肾功能正常。1只对照狗死于尿毒症，在其余4只中观察到短暂性肾衰竭。在两种不同动物身上进行的这些实验突出了在缺血期后给予二甲基亚砜时其对缺血肾脏的保护作用。
