Montgomery K T, Biedler J L, Spengler B A, Melera P W
Proc Natl Acad Sci U S A. 1983 Sep;80(18):5724-8. doi: 10.1073/pnas.80.18.5724.
Southern blot analysis of a number of EcoRI-digested human neuroblastoma DNAs has revealed the presence of a family of discrete restriction fragments, the majority of which are amplified in most, but not all, of the neuroblastoma cell lines tested. None of these sequences is abundantly present in DNA from other human tumors of different tissue origins, including several either known or presumed to contain amplified DNA. Hence, these sequences appear to be specifically amplified by neuroblastoma cells. Hybridization with metaphase chromosomes in situ has localized these sequences to either the homogeneously staining regions or double-minute chromosomes of different neuroblastoma cell lines, indicating that these chromosomal structures, although present in cell lines established from different patients, share many sequences and may have a common, but as yet unknown, function.
对多个经EcoRI酶切的人神经母细胞瘤DNA进行Southern印迹分析，结果显示存在一组离散的限制性片段家族，其中大多数在大多数（但并非全部）测试的神经母细胞瘤细胞系中呈扩增状态。在来自不同组织起源的其他人类肿瘤的DNA中，这些序列均未大量存在，包括几种已知或推测含有扩增DNA的肿瘤。因此，这些序列似乎是由神经母细胞瘤细胞特异性扩增的。与中期染色体进行原位杂交已将这些序列定位到不同神经母细胞瘤细胞系的均匀染色区或双微体染色体上，这表明这些染色体结构尽管存在于从不同患者建立的细胞系中，但共享许多序列，并且可能具有一个共同的、但尚未明确的功能。
