Shimizu E, Saijo N, Shibuya M, Takizawa T, Fujioka Y, Hoshi A
Gan To Kagaku Ryoho. 1983 Sep;10(9):2030-5.
The effects of various biological response modifiers (BRM) such as Nocardia rubra cell wall skeleton (N-CWS), OK-432, muramyl dipeptide (MDP), Staphylococcal protein A, interferon, lymphokine on the cytostatic activity of granulocytes against cultured tumor cells were examined. The cytostatic activity of untreated granulocytes from 10 normal healthy volunteers ranged from 14% to 30%. Lymphokine obtained from the supernatant of lymphocytes cultured with Con A-Sepharose directly suppressed the DNA synthesis of K 562 cells in high concentration, however, other BRM showed no direct cytostatic activity. The cytostatic activity of granulocytes was augmented by low concentrations (2.5 micrograms/ml) of N-CWS, OK-432 and protein A as well as by high concentration (25 micrograms/ml) of MDP. However, the cytostatic activity was not influenced by alpha-interferon. The meanings and mechanisms of the elevation in cytostatic activity of granulocytes by BRM were discussed with regards to the clinical immunotherapy.
研究了多种生物反应调节剂（BRM），如红色诺卡氏菌细胞壁骨架（N-CWS）、OK-432、胞壁酰二肽（MDP）、葡萄球菌蛋白A、干扰素、淋巴因子对粒细胞针对培养肿瘤细胞的细胞抑制活性的影响。10名正常健康志愿者未经处理的粒细胞的细胞抑制活性范围为14%至30%。用Con A-琼脂糖培养淋巴细胞的上清液获得的淋巴因子在高浓度下直接抑制K 562细胞的DNA合成，然而，其他BRM未显示出直接的细胞抑制活性。低浓度（2.5微克/毫升）的N-CWS、OK-432和蛋白A以及高浓度（25微克/毫升）的MDP可增强粒细胞的细胞抑制活性。然而，α-干扰素对细胞抑制活性没有影响。就临床免疫治疗而言，讨论了BRM提高粒细胞细胞抑制活性的意义和机制。
