Yanagawa T, Oguro M, Takagi T, Takenaga K
Gan To Kagaku Ryoho. 1984 Oct;11(10):2155-62.
Although the role of biological response modifiers in cancer therapy is well recognised, their mechanism of action remains unsettled. It is empirically considered that there may be some direct antitumor effects induced by some BRM. To analyse the mechanism of action, we evaluated the effects of BRM by using our in vitro sensitivity test, the principle of which was based on dynamic and morphological observation of target cells in vitro. Five kinds of BRM (OK-432, PSK, N-CWS, Bestatin, Lentinan) were tested and the target cell lines were L1210 and P388. The results showed that OK-432 and PSK were mildly cytotoxic, and that OK-432, PSK and Bestatin were effective for cell growth suppression.
尽管生物反应调节剂在癌症治疗中的作用已得到充分认可，但其作用机制仍未明确。根据经验推测，某些生物反应调节剂可能会产生一些直接的抗肿瘤作用。为了分析其作用机制，我们通过体外敏感性试验评估了生物反应调节剂的作用，该试验的原理基于对体外靶细胞的动态和形态学观察。测试了五种生物反应调节剂（溶链菌制剂、云芝多糖K、奴卡菌细胞壁骨架、抑氨肽酶B、香菇多糖），靶细胞系为L1210和P388。结果显示，溶链菌制剂和云芝多糖K具有轻度细胞毒性，且溶链菌制剂、云芝多糖K和抑氨肽酶B对细胞生长抑制有效。
