Ferrero D, Gallo E, Lanfrancone L, Tarella C
Exp Cell Res. 1983 Aug;147(1):111-8. doi: 10.1016/0014-4827(83)90275-6.
Two HL60 clones (C12 and C13) totally insensitive to differentiation induction by dimethylsulfoxide (Me2SO) are described. They have been growing continuously in the presence of the inducer for more than 6 months. The morphological and cytochemical features of the two populations are quite similar to those of the original HL60 cell line, whereas a different karyotype with marked hyperploidy (modal chromosome number of 86 for C12 and 82 for C13) was detected. An antigenic pattern analogous to that of the native HL60 cell line was found in C12 and C13 populations using three monoclonal antibodies differently reactive to myeloid cells. Both clones can be induced to differentiate by retinoic acid (RA) and 12-O-tetradecanoylphorbol 13-acetate (TPA). The pattern of differentiation was assessed by morphological, cytochemical, phenotypical and functional markers. Differentiation of C12 cells by RA and TPA was similar to that observed with native HL60 cells, whereas C13 cells showed lower degrees of sensitivity to RA and TPA. The data presented suggest the existence of different mechanisms for induction of differentiation by Me2SO, RA and TPA. In addition, they are in accordance with previous observations of different degrees of inducibility to differentiation among leukemic cell populations in culture.
本文描述了两个对二甲基亚砜（Me2SO）诱导分化完全不敏感的HL60克隆（C12和C13）。它们在诱导剂存在的情况下已连续生长超过6个月。这两个细胞群体的形态学和细胞化学特征与原始HL60细胞系非常相似，然而检测到一种具有明显超倍体的不同核型（C12的众数染色体数为86，C13为82）。使用三种对髓系细胞反应性不同的单克隆抗体，在C12和C13群体中发现了与天然HL60细胞系类似的抗原模式。两个克隆都可被视黄酸（RA）和12 - O - 十四烷酰佛波醇13 - 乙酸酯（TPA）诱导分化。通过形态学、细胞化学、表型和功能标记物评估分化模式。RA和TPA诱导C12细胞分化的情况与天然HL60细胞相似，而C13细胞对RA和TPA的敏感性较低。所呈现的数据表明，Me2SO、RA和TPA诱导分化存在不同机制。此外，它们与之前关于培养的白血病细胞群体中不同程度分化诱导性的观察结果一致。
