Ferrero D, Pessano S, Pagliardi G L, Rovera G
Blood. 1983 Jan;61(1):171-9.
The surface changes occurring in three acute myeloid leukemia cell lines (HL60, ML3, and KG1) induced to differentiate by a variety of agents (dimethylsulfoxide, retinoic acid, 12-O-tetradecanoylphorbol-13-acetate, and factors present in lymphocyte conditioned medium) were probed using monoclonal antibodies that are differentiation stage- and lineage-specific. In all cases, the differentiated phenotype was defective and varied with the inducing agent and the cell line used. HL60 proved to be the most sensitive to the effect of the inducers. Retinoic acid was better than DMSO, and TPA was better than the medium factors in the ability to induce granulocytic and monocytic differentiation, respectively, in HL60 cells. These findings indicate that the differentiation block in acute myeloid leukemias is heterogeneous and that each cell line has different phenotypic characteristics that are responsible for the extent of differentiation obtained with a given inducer. These results also suggest that the extent of the differentiation response in vitro may be improved by the use of more suitable inducers for each specific leukemic line.
使用针对分化阶段和谱系具有特异性的单克隆抗体，探究了三种急性髓系白血病细胞系（HL60、ML3和KG1）在多种诱导剂（二甲亚砜、视黄酸、12-O-十四烷酰佛波醇-13-乙酸酯以及淋巴细胞条件培养基中的因子）作用下发生的表面变化。在所有情况下，分化表型均有缺陷，且因诱导剂和所用细胞系而异。结果表明，HL60对诱导剂的作用最为敏感。视黄酸在诱导HL60细胞向粒细胞分化方面优于二甲亚砜，而12-O-十四烷酰佛波醇-13-乙酸酯在诱导HL60细胞向单核细胞分化方面优于培养基中的因子。这些发现表明，急性髓系白血病中的分化阻滞是异质性的，且每个细胞系都有不同的表型特征，这些特征决定了用给定诱导剂获得的分化程度。这些结果还表明，通过为每种特定白血病细胞系使用更合适的诱导剂，体外分化反应的程度可能会得到提高。
