Nordgren I K, Forney R B, Carroll F T, Holmstedt B R, Jäderholm-Ek I, Pettersson B M
Arch Toxicol Suppl. 1983;6:339-50. doi: 10.1007/978-3-642-69083-9_63.
The neuromuscular blocking agent succinylcholine (SCh) has been identified and quantitated in biological material using gas chromatography-mass spectrometry. The bisquaternary ammonium compound SCh is extracted from tissue homogenates or body fluids into dichloromethane as an ion pair with hexanitrodiphenylamine (DPA). The evaporated ion pair residue is demethylated with sodium benzenethiolate to form the corresponding tertiary amine which is identified and quantitated by gas chromatography-mass spectrometry using a glass capillary column coated with SE 52. In the quantitative analysis deuterated SCh is used as internal standard. The instrument is focussed on m/z 58 for demethylated SCh and m/z 62 or 64 for the internal standard. Concentrations as low as 5 ng SCh iodide/g tissue or body fluid are easily detected.
已使用气相色谱 - 质谱法对生物材料中的神经肌肉阻滞剂琥珀酰胆碱（SCh）进行了鉴定和定量。双季铵化合物SCh作为与六硝基二苯胺（DPA）的离子对从组织匀浆或体液中萃取到二氯甲烷中。蒸发后的离子对残留物用苯硫酚钠进行脱甲基反应，形成相应的叔胺，然后使用涂有SE 52的玻璃毛细管柱通过气相色谱 - 质谱法进行鉴定和定量。在定量分析中，氘代SCh用作内标。仪器聚焦于脱甲基化SCh的m/z 58和内标的m/z 62或64。低至5 ng碘化SCh/g组织或体液的浓度都很容易检测到。
