Bunnett N W, Walsh J H, Debas H T, Kauffman G L, Golanska E M
Gastroenterology. 1983 Dec;85(6):1391-8.
The purpose of the study was to develop a method for collecting interstitial fluid bathing the stomach tissues in which prostaglandins could be measured. Hollow dialysis fibers attached at the ends to Silastic tubes were surgically implanted into the submucosa of the gastric fundus and antrum of dogs. The Silastic tubes were exteriorized through the body wall. After full recovery from surgery, the fibers were filled with 5% bovine serum albumin in isotonic saline that was replaced at 5-min intervals. Prostaglandin E2 was measured in the dialysate by radioimmunoassay. In 6 dogs, feeding significantly stimulated the release of prostaglandin E2 into the fundic interstitial fluid from 5.3 +/- 0.6 ng X ml-1 to 12.1 +/- 1.6 ng X ml-1 (p less than 0.01) but had no effect on antral levels. In 4 dogs, indomethacin (0.01, 0.1, 1.0, and 10.0 ng X kg-1, i.v.) caused a dose-dependent depression in prostaglandin E2 levels in interstitial fluid of the fundus and antrum. In 4 other dogs, indomethacin depressed the ex vivo generation of prostaglandin E2 in biopsy specimens of the fundus and antrum. These results validate the technique of interstitial fluid dialysis and suggest that it is a powerful method for examining the secretion of locally acting substances in the stomach of conscious animals.
本研究的目的是开发一种收集胃组织周围间质液的方法，以便能够测量其中的前列腺素。将两端连接硅橡胶管的中空透析纤维通过手术植入狗胃底和胃窦的黏膜下层。硅橡胶管经腹壁引出。手术完全恢复后，向纤维中注入含5%牛血清白蛋白的等渗盐水，并每隔5分钟更换一次。通过放射免疫分析法测定透析液中的前列腺素E2。在6只狗中，进食显著刺激前列腺素E2从胃底间质液中的释放，从5.3±0.6 ng·ml-1升至12.1±1.6 ng·ml-1（p<0.01），但对胃窦水平无影响。在4只狗中，静脉注射吲哚美辛（0.01、0.1、1.0和10.0 ng·kg-1）导致胃底和胃窦间质液中前列腺素E2水平呈剂量依赖性降低。在另外4只狗中，吲哚美辛抑制了胃底和胃窦活检标本中前列腺素E2的体外生成。这些结果验证了间质液透析技术，并表明它是一种用于检测清醒动物胃中局部作用物质分泌的有效方法。
