Seiler K, Seiler R, Claus R, Sterba G
Gen Comp Endocrinol. 1983 Sep;51(3):353-63. doi: 10.1016/0016-6480(83)90050-3.
Hydroxysteroid dehydrogenase (HSD) activity was determined spectrophotometrically on homogenates of the pronephros, the opisthonephros, and the gonads of metamorphosing, immature, and mature adult male and female brook lampreys (Lampetra planeri Bloch), employing pregnenolone, dehydroepiandrosterone, androsterone, epiandrosterone, and 3 beta,17 beta-dihydroxy-5 alpha-androstane as substrates and NAD or NADP as coenzymes in October, December, February, and April. With NADP a higher HSD-dependent HSD activity was detected in presumed adrenocortical tissue (PAT) homogenates with pregnenolone and 3 beta,17 beta-dihydroxy-5 alpha-androstane and in gonad homogenates only with 3 beta,17 beta-dihydroxy-5 alpha-androstane. With 3 beta,17 beta-dihydroxy-5 alpha-androstane as substrate the highest enzyme activities were measured in PAT and gonad homogenates from animals of all investigated life stages. A varying intensity of HSD activity in the examined tissue homogenates probably dependent on stages of the individual development was observed.
在10月、12月、2月和4月，以孕烯醇酮、脱氢表雄酮、雄酮、表雄酮和3β,17β - 二羟基 - 5α - 雄烷为底物，以NAD或NADP为辅酶，采用分光光度法测定了变态期、未成熟期和成熟成年雌雄溪七鳃鳗（Lampetra planeri Bloch）的前肾、后肾和性腺匀浆中的羟基类固醇脱氢酶（HSD）活性。使用NADP时，在假定的肾上腺皮质组织（PAT）匀浆中，以孕烯醇酮和3β,17β - 二羟基 - 5α - 雄烷为底物时检测到较高的HSD依赖性HSD活性，而在性腺匀浆中仅以3β,17β - 二羟基 - 5α - 雄烷为底物时检测到较高活性。以3β,17β - 二羟基 - 5α - 雄烷为底物时，在所有研究生命阶段动物的PAT和性腺匀浆中测得最高的酶活性。在所检查的组织匀浆中观察到HSD活性强度不同，这可能取决于个体发育阶段。
